Dual infection with human immunodeficiency virus type 1 of distinct envelope subtypes in humans

Andrew W. Artenstein, Thomas C. Van Cott, John R. Mascola, Jean K. Carr, Patricia A. Hegerich, Jariyanart Gaywee, Eric Sanders-Buell, Merlin L. Robb, Debora E. Dayhoff, Sataporn Thitivichianlert, Sorachai Nitayaphan, John G. Mc Neil, Deborah L. Birx, Rodney A. Michael, Donald S. Burke, Francine E. Mc Cutchan

Research output: Contribution to journalArticlepeer-review

131 Scopus citations


Multiple genetic subtypes of human immunodeficiency virus type 1 (HIV-1) have been identified among internationally collected isolates. The HIV-1 epidemic in Thailand is largely due to B and E subtypes of virus. Dual infection with distinct HIV-1 subtypes would suggest that antiviral immunity evoked by one subtype can be incompletely protective against a second. Polymerase chain reaction typing and serologic typing were used to screen a panel of specimens from HIV-1infected subjects in Thailand. Two persons simultaneously harbored HIV-1 of env subtypes B and E, and this was confirmed by colony hybridization with subtype-specific probes and nucleotide sequence analysis of a 630-bp fragment of gp120 from multiple molecular clones. In addition, both subtypes were identified in cocultured peripheral blood mononuclear cells from 1 individual. These data provide the first evidence of dual HIV-1 infection in humans and reinforce the need for polyvalent vaccines.

Original languageEnglish (US)
Pages (from-to)805-810
Number of pages6
JournalJournal of Infectious Diseases
Issue number4
StatePublished - Apr 1995
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


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