Abstract
We describe a dual-color three-dimensional stimulated emission depletion (3D-STED) microscopy employing a single laser source with a repetition rate of 80 MHz. Multiple excitation pulses synchronized with a STED pulse were generated by a photonic crystal fiber, and the desired wavelengths were selected by an acousto-optic tunable filter with high spectral purity. Selective excitation at different wavelengths permits simultaneous imaging of two fluorescent markers at a nanoscale resolution in three dimensions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2653-2656 |
| Number of pages | 4 |
| Journal | Optics Letters |
| Volume | 40 |
| Issue number | 11 |
| DOIs | |
| State | Published - Jun 1 2015 |
| Externally published | Yes |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics