Down-regulation of AMPA receptor subunit GluR2 in amygdaloid kindling

Heather K. Prince, P. Jeffrey Conn, Craig D. Blackstone, Rick L. Huganir, Allan I. Levey

Research output: Contribution to journalArticlepeer-review

78 Scopus citations


Alterations in glutamatergic transmission are postulated to be important in kindling and epilepsy. The levels of α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid (AMPA) receptor subunits (GluR1, 2, and 4) were compared in amygdala-kindled and sham-operated animals using subunit-specific antibodies and quantitative western blotting. Four limbic regions were examined: limbic forebrain, piriform cortex/amygdala, hip- pocampus, and entorhinal cortex. When subunit levels were examined 24 h after the last stage 5 seizure, levels of GluR2 were found to be selectively reduced in limbic forebrain (30%) and piriform cortex/amygdala (25%), with no changes in other regions examined. In addition, no changes in the other subunits were observed in any region. The decrease in GluR2 that was observed in kindled animals at 24 h was no longer present at 1 week and 1 month after the last stage 5 seizure. Because the GluR2 subunit uniquely determines the calcium permeability of these receptors and because the piriform cortex has been implicated as a source of excitatory drive for limbic seizures, reduced GluR2 expression may be important in increasing neuronal excitability in kindling-induced epilepsy, or may reflect a compensatory mechanism resulting from kindling.

Original languageEnglish (US)
Pages (from-to)462-465
Number of pages4
JournalJournal of Neurochemistry
Issue number1
StatePublished - Jan 1995


  • Glutamate receptors
  • Kindling
  • Piriform cortex

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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