Dopamine transporter mRNA: dense expression in ventral midbrain neurons

Shoichi Shimada; Shigeo Kitayama; Donna Walther; George Uhl

doi:10.1016/0169-328X(92)90220-6

Dopamine transporter mRNA: dense expression in ventral midbrain neurons

Shoichi Shimada, Shigeo Kitayama, Donna Walther, George Uhl

School of Medicine

Research output: Contribution to journal › Article › peer-review

117 Scopus citations

Abstract

Oligonucleotides and a full-length cDNA encoding a functional dopamine transporter (DAT1) hybridize to a 3.7 kb mRNA that is concentrated in mRNA prepared from midbrain and absent in specimens from cerebellum or cerebral cortex. In situ hybridization reveals substantial hybridization densities overlying neurons of the substantia nigra, pars compacta, and the parabrachialis pigmentosus region of the ventral tegmental area (VTA). Neurons in the linear and paranigral VTA regions display lower levels of expression. Preliminary studies in arcuate neurons suggest modest hybridization. Different dopaminergic cell groups display different levels of DAT1 dopamine transporter expression.

Original language	English (US)
Pages (from-to)	359-362
Number of pages	4
Journal	Molecular Brain Research
Volume	13
Issue number	4
DOIs	https://doi.org/10.1016/0169-328X(92)90220-6
State	Published - 1992

Keywords

Dopamine transporter mRNA
Substantia nigra
Ventral tegmental area

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/0169-328X(92)90220-6

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abstract = "Oligonucleotides and a full-length cDNA encoding a functional dopamine transporter (DAT1) hybridize to a 3.7 kb mRNA that is concentrated in mRNA prepared from midbrain and absent in specimens from cerebellum or cerebral cortex. In situ hybridization reveals substantial hybridization densities overlying neurons of the substantia nigra, pars compacta, and the parabrachialis pigmentosus region of the ventral tegmental area (VTA). Neurons in the linear and paranigral VTA regions display lower levels of expression. Preliminary studies in arcuate neurons suggest modest hybridization. Different dopaminergic cell groups display different levels of DAT1 dopamine transporter expression.",

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T1 - Dopamine transporter mRNA

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AU - Shimada, Shoichi

AU - Kitayama, Shigeo

AU - Walther, Donna

AU - Uhl, George

PY - 1992

Y1 - 1992

N2 - Oligonucleotides and a full-length cDNA encoding a functional dopamine transporter (DAT1) hybridize to a 3.7 kb mRNA that is concentrated in mRNA prepared from midbrain and absent in specimens from cerebellum or cerebral cortex. In situ hybridization reveals substantial hybridization densities overlying neurons of the substantia nigra, pars compacta, and the parabrachialis pigmentosus region of the ventral tegmental area (VTA). Neurons in the linear and paranigral VTA regions display lower levels of expression. Preliminary studies in arcuate neurons suggest modest hybridization. Different dopaminergic cell groups display different levels of DAT1 dopamine transporter expression.

AB - Oligonucleotides and a full-length cDNA encoding a functional dopamine transporter (DAT1) hybridize to a 3.7 kb mRNA that is concentrated in mRNA prepared from midbrain and absent in specimens from cerebellum or cerebral cortex. In situ hybridization reveals substantial hybridization densities overlying neurons of the substantia nigra, pars compacta, and the parabrachialis pigmentosus region of the ventral tegmental area (VTA). Neurons in the linear and paranigral VTA regions display lower levels of expression. Preliminary studies in arcuate neurons suggest modest hybridization. Different dopaminergic cell groups display different levels of DAT1 dopamine transporter expression.

KW - Dopamine transporter mRNA

KW - Substantia nigra

KW - Ventral tegmental area

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Dopamine transporter mRNA: dense expression in ventral midbrain neurons

Abstract

Keywords

ASJC Scopus subject areas

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