Abstract
We have observed evidence of increased telomere shortening in short-term T-lymphocyte cultures following freezing and thawing of the original inoculum obtained by ficoll-paque gradient centrifugation, compared to T-lymphocytes that were cultured immediately without freezing and thawing from the same blood sample from 3 female and 3 male adults. Because freezing may have similar effects on other cell types, and because telomere shortening may only manifest its effects after many years or decades, we suggest there is a pressing need for evaluation of the effects of freezing on any cells envisioned for clinical applications, including embryo implantation.
Original language | English (US) |
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Pages (from-to) | 72-73 |
Number of pages | 2 |
Journal | Cryobiology |
Volume | 65 |
Issue number | 1 |
DOIs | |
State | Published - Aug 2012 |
Externally published | Yes |
Keywords
- Cryogenics
- Embryos
- FISH
- PNA probe
- Short-term cultures
- Stem cells
- T-lymphocytes
- Telomere " loss"
- Telomere shortening
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- General Agricultural and Biological Sciences