TY - JOUR
T1 - Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?
AU - de Morais, Pedro Henrique Alves
AU - Silva, Rafael Francisco Alves
AU - Ribeiro, Thiago da Silva
AU - de Farias, Igor Eduardo Caetano
AU - Lino Junior, Ruy de Souza
AU - Carneiro, Fabiana Pirani
AU - Durães, Leonardo de Castro
AU - de Sousa, João Batista
N1 - Publisher Copyright:
© 2020, Sociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia. All rights reserved.
PY - 2020
Y1 - 2020
N2 - Purpose: To determine by histomorphometric analysis whether CO2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods: This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE: 30 min preoperatively; POST: 30 min postoperatively; PP: 30 min pre-and postoperatively; C: control group). CO2 pneumoperitoneumwas insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results: There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion: At 5 mmHg, CO2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats.
AB - Purpose: To determine by histomorphometric analysis whether CO2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods: This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE: 30 min preoperatively; POST: 30 min postoperatively; PP: 30 min pre-and postoperatively; C: control group). CO2 pneumoperitoneumwas insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results: There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion: At 5 mmHg, CO2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats.
KW - Collagen
KW - Incisional Hernia
KW - Laparoscopy
KW - Minimally Invasive Surgical Procedures
KW - Pneumoperitoneum
KW - Rats
KW - Wound Healing
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U2 - 10.1590/s0102-865020200060000005
DO - 10.1590/s0102-865020200060000005
M3 - Article
C2 - 32667584
AN - SCOPUS:85087873979
SN - 0102-8650
VL - 35
SP - 1
EP - 6
JO - Acta cirurgica brasileira
JF - Acta cirurgica brasileira
IS - 6
M1 - e202000605
ER -