Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?

Pedro Henrique Alves de Morais, Rafael Francisco Alves Silva, Thiago da Silva Ribeiro, Igor Eduardo Caetano de Farias, Ruy de Souza Lino Junior, Fabiana Pirani Carneiro, Leonardo de Castro Durães, João Batista de Sousa

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1 Scopus citations


Purpose: To determine by histomorphometric analysis whether CO2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods: This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE: 30 min preoperatively; POST: 30 min postoperatively; PP: 30 min pre-and postoperatively; C: control group). CO2 pneumoperitoneumwas insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results: There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion: At 5 mmHg, CO2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats.

Original languageEnglish (US)
Article numbere202000605
Pages (from-to)1-6
Number of pages6
JournalActa cirurgica brasileira
Issue number6
StatePublished - 2020


  • Collagen
  • Incisional Hernia
  • Laparoscopy
  • Minimally Invasive Surgical Procedures
  • Pneumoperitoneum
  • Rats
  • Wound Healing

ASJC Scopus subject areas

  • Surgery


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