TY - JOUR
T1 - DNA TYPING FOR HLA‐DPB1‐ALLELES IN GERMAN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS USING THE POLYMERASE CHAIN REACTION AND DIG‐ddUTP‐LABELLED OLIGONUCLEOTIDE PROBES
AU - The Members of The SLE Study Group
AU - Yao, Z.
AU - Hartung, K.
AU - Deicher, H. G.
AU - Brunnler, G.
AU - Bettinotti, M. P.
AU - Keller, E.
AU - Paul, C.
AU - Gawron, C.
AU - Mikschl, S.
AU - Albert, E.
PY - 1993/8
Y1 - 1993/8
N2 - Genomic DNA of 178 German Caucasian patients with systemic lupus erythematosus are studied for HLA‐DP locus by using PCR and DIG‐ddUTP‐labelled oligonucleotide probes. A significant increase of DPB1*0101 is observed in SLE patients compared with healthy controls (χ < 2 < = 15.27, p.c. <0.004). DPB1*0501 and *0901 are also slightly increased (χ2= 5.85, P < 0.05, p.c. = NS; χ2= 5.64, P < 0.05, p.c. = NS). There is no significant difference in frequency of DP alleles between male and female patients. Since a linkage disequilibrium between HLA‐B, DR and DP loci is found in our SLE patients, an analysis is performed assessing the relative importance of these HLA‐markers to SLE. The results show that the increase of DPB1*0101 in SLE patients is associated with the HLA‐B8, DR3 haplotype and it suggests a more important role for HLA‐B8, DR3 or genes within this haplotype than for DPB1*0101 in the genetic predisposition for SLE.
AB - Genomic DNA of 178 German Caucasian patients with systemic lupus erythematosus are studied for HLA‐DP locus by using PCR and DIG‐ddUTP‐labelled oligonucleotide probes. A significant increase of DPB1*0101 is observed in SLE patients compared with healthy controls (χ < 2 < = 15.27, p.c. <0.004). DPB1*0501 and *0901 are also slightly increased (χ2= 5.85, P < 0.05, p.c. = NS; χ2= 5.64, P < 0.05, p.c. = NS). There is no significant difference in frequency of DP alleles between male and female patients. Since a linkage disequilibrium between HLA‐B, DR and DP loci is found in our SLE patients, an analysis is performed assessing the relative importance of these HLA‐markers to SLE. The results show that the increase of DPB1*0101 in SLE patients is associated with the HLA‐B8, DR3 haplotype and it suggests a more important role for HLA‐B8, DR3 or genes within this haplotype than for DPB1*0101 in the genetic predisposition for SLE.
UR - http://www.scopus.com/inward/record.url?scp=0027199294&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027199294&partnerID=8YFLogxK
U2 - 10.1111/j.1744-313X.1993.tb00141.x
DO - 10.1111/j.1744-313X.1993.tb00141.x
M3 - Article
C2 - 8399121
AN - SCOPUS:0027199294
SN - 1744-3121
VL - 20
SP - 259
EP - 266
JO - International Journal of Immunogenetics
JF - International Journal of Immunogenetics
IS - 4
ER -