TY - JOUR
T1 - Dna damage caused by reactive oxygen species originating from a copper-dependent oxidation of the 2-hydroxy catechol of estradiol
AU - Li, Yunbo
AU - Trush, Michael A.
AU - Yager, James D.
N1 - Funding Information:
The authors wish to acknowledge support from US Public Health Service grants NCI CA 36701 and ES 05131. Core facilities and shared-use equipment were supported by NIEHS core grant ES 03819.
PY - 1994/7
Y1 - 1994/7
N2 - It has previously been proposed that redox cycling between catechol estrogens and their quinones, mediated by cytochrome P450, could lead to the generation of free radicals that would subsequently cause oxidative damage to DNA and proteinsthat might have a role in hormonal carcinogenesis. Alternative, non-enzymatic mechanisms involving copper have been shown to participate in the oxidation of various chemicals through processes that also result in the appearance of reactive oxygen species and subsequent site-specific oxidative DNA damage. The goal of the present study was to determine whether the 2-hydroxy-catechol of estradiol (2-OH-E2) can be oxidized by copper through a process which generates reactive oxygen species that cause oxidative DNA damage as detected by the appearance of strand breaks in πX-174 plasmid DNA. Our results show that both single- and double-strand breaks are formed in the presence of Cu(II) plus micromolar concentrations of 2-OH-E2, and 4-OH-E2, in a concentration/time-dependent process. No strand breaks were detected in the presence ofCu(II) or 2-OH-E2 alone. The reaction of 2-OH-E2 with Cu(II) was accompanied by the reduction of Cu(II) to Cu(I), the utilization of O2, and the generation of H2O2. The utilization of O2 and the formation of strand breaks was completely blocked by the Cu(I)-specific chelator bathocuproinedisul-fonic acid (BCS) at a ratio of BCS to Cu(II) of 4: 1. The appearance of strand breaks was also blocked by catalase and inhibited by the singlet oxygen scavengers sodium azide and 2, 2, 6, 6-tetramethyl-4-piperidone. In contrast the free hydroxyl radical scavengers mannitol and N-tert-butyl-α-phenylnitrone were not effective inhibitors; superoxide dismutase had no inhibitory effect. These results are similar to what has been observed by others for the formation of oxidative DNA damage by the H2O2/Cu(II) system and by us for the induction of strand breaks by hydroquinone/ Cu(II). Since copper is known to be present in the nucleus, particularly in association with guanines in DNA, our results with 2-OH-E2/Cu(II) together with those of others with H2O2/Cu(II), discussed below, suggest an alternate site-specific mechanism for the formation of oxidative DNA damage associated with estrogen treatment. Furthermore, the results suggest that the oxidative damage results from the localized generation of singlet oxygen or a similar bound reactive entity rather than free hydroxyl radical.
AB - It has previously been proposed that redox cycling between catechol estrogens and their quinones, mediated by cytochrome P450, could lead to the generation of free radicals that would subsequently cause oxidative damage to DNA and proteinsthat might have a role in hormonal carcinogenesis. Alternative, non-enzymatic mechanisms involving copper have been shown to participate in the oxidation of various chemicals through processes that also result in the appearance of reactive oxygen species and subsequent site-specific oxidative DNA damage. The goal of the present study was to determine whether the 2-hydroxy-catechol of estradiol (2-OH-E2) can be oxidized by copper through a process which generates reactive oxygen species that cause oxidative DNA damage as detected by the appearance of strand breaks in πX-174 plasmid DNA. Our results show that both single- and double-strand breaks are formed in the presence of Cu(II) plus micromolar concentrations of 2-OH-E2, and 4-OH-E2, in a concentration/time-dependent process. No strand breaks were detected in the presence ofCu(II) or 2-OH-E2 alone. The reaction of 2-OH-E2 with Cu(II) was accompanied by the reduction of Cu(II) to Cu(I), the utilization of O2, and the generation of H2O2. The utilization of O2 and the formation of strand breaks was completely blocked by the Cu(I)-specific chelator bathocuproinedisul-fonic acid (BCS) at a ratio of BCS to Cu(II) of 4: 1. The appearance of strand breaks was also blocked by catalase and inhibited by the singlet oxygen scavengers sodium azide and 2, 2, 6, 6-tetramethyl-4-piperidone. In contrast the free hydroxyl radical scavengers mannitol and N-tert-butyl-α-phenylnitrone were not effective inhibitors; superoxide dismutase had no inhibitory effect. These results are similar to what has been observed by others for the formation of oxidative DNA damage by the H2O2/Cu(II) system and by us for the induction of strand breaks by hydroquinone/ Cu(II). Since copper is known to be present in the nucleus, particularly in association with guanines in DNA, our results with 2-OH-E2/Cu(II) together with those of others with H2O2/Cu(II), discussed below, suggest an alternate site-specific mechanism for the formation of oxidative DNA damage associated with estrogen treatment. Furthermore, the results suggest that the oxidative damage results from the localized generation of singlet oxygen or a similar bound reactive entity rather than free hydroxyl radical.
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U2 - 10.1093/carcin/15.7.1421
DO - 10.1093/carcin/15.7.1421
M3 - Article
C2 - 8033320
AN - SCOPUS:0028237181
SN - 0143-3334
VL - 15
SP - 1421
EP - 1427
JO - Carcinogenesis
JF - Carcinogenesis
IS - 7
ER -