DNA content measurements and an improved idiogram for the Indian muntjac

Howard P. Levy; Roger A. Schultz; José V. Ordonez; Maimon M. Cohen

doi:10.1002/cyto.990140404

DNA content measurements and an improved idiogram for the Indian muntjac

Howard P. Levy, Roger A. Schultz, José V. Ordonez, Maimon M. Cohen

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The Indian muntjac, an asiatic deer, has the lowest diploid chromosome number among mammals (female 2N = 6; male 2N = 7). Using flow cytometric quantification of propidium iodide‐stained cells, we determined the DNA content of muntjac cells to be 94% that of human. This suggests that the muntjac may serve as a model for investigation of karyotypic evolution and rearrangement. In order to facilitate future comparative gene mapping studies, computer‐aided analysis of digitized metaphase chromosomes allowed development of a detailed Indian muntjac G‐banded idiogram incorporating both ISCN‐type nomenclature and quantitative estimates of the size of each band and position. © 1993 Wiley‐Liss, Inc.

Original language	English (US)
Pages (from-to)	362-368
Number of pages	7
Journal	Cytometry
Volume	14
Issue number	4
DOIs	https://doi.org/10.1002/cyto.990140404
State	Published - 1993
Externally published	Yes

Keywords

Cellular DNA content
G‐band idiogram
image analysis

ASJC Scopus subject areas

Pathology and Forensic Medicine
Biophysics
Hematology
Endocrinology
Cell Biology

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10.1002/cyto.990140404

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title = "DNA content measurements and an improved idiogram for the Indian muntjac",

abstract = "The Indian muntjac, an asiatic deer, has the lowest diploid chromosome number among mammals (female 2N = 6; male 2N = 7). Using flow cytometric quantification of propidium iodide‐stained cells, we determined the DNA content of muntjac cells to be 94% that of human. This suggests that the muntjac may serve as a model for investigation of karyotypic evolution and rearrangement. In order to facilitate future comparative gene mapping studies, computer‐aided analysis of digitized metaphase chromosomes allowed development of a detailed Indian muntjac G‐banded idiogram incorporating both ISCN‐type nomenclature and quantitative estimates of the size of each band and position. {\textcopyright} 1993 Wiley‐Liss, Inc.",

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T1 - DNA content measurements and an improved idiogram for the Indian muntjac

AU - Levy, Howard P.

AU - Schultz, Roger A.

AU - Ordonez, José V.

AU - Cohen, Maimon M.

PY - 1993

Y1 - 1993

N2 - The Indian muntjac, an asiatic deer, has the lowest diploid chromosome number among mammals (female 2N = 6; male 2N = 7). Using flow cytometric quantification of propidium iodide‐stained cells, we determined the DNA content of muntjac cells to be 94% that of human. This suggests that the muntjac may serve as a model for investigation of karyotypic evolution and rearrangement. In order to facilitate future comparative gene mapping studies, computer‐aided analysis of digitized metaphase chromosomes allowed development of a detailed Indian muntjac G‐banded idiogram incorporating both ISCN‐type nomenclature and quantitative estimates of the size of each band and position. © 1993 Wiley‐Liss, Inc.

AB - The Indian muntjac, an asiatic deer, has the lowest diploid chromosome number among mammals (female 2N = 6; male 2N = 7). Using flow cytometric quantification of propidium iodide‐stained cells, we determined the DNA content of muntjac cells to be 94% that of human. This suggests that the muntjac may serve as a model for investigation of karyotypic evolution and rearrangement. In order to facilitate future comparative gene mapping studies, computer‐aided analysis of digitized metaphase chromosomes allowed development of a detailed Indian muntjac G‐banded idiogram incorporating both ISCN‐type nomenclature and quantitative estimates of the size of each band and position. © 1993 Wiley‐Liss, Inc.

KW - Cellular DNA content

KW - G‐band idiogram

KW - image analysis

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DNA content measurements and an improved idiogram for the Indian muntjac

Abstract

Keywords

ASJC Scopus subject areas

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