Diverse influences of androgen-disrupting chemicals on immune responses mounted by macrophages

Kyong Hoon Kim; Seung Min Yeon; Hyun Gyung Kim; Hyun Suk Choi; Hyojeung Kang; Hee Deung Park; Tae Won Park; Seung Pil Pack; Eun Hee Lee; Youngjoo Byun; Sang Eun Choi; Kenneth Sung Lee; Un Hwan Ha; Yong Woo Jung

doi:10.1007/s10753-013-9781-1

Diverse influences of androgen-disrupting chemicals on immune responses mounted by macrophages

Kyong Hoon Kim, Seung Min Yeon, Hyun Gyung Kim, Hyun Suk Choi, Hyojeung Kang, Hee Deung Park, Tae Won Park, Seung Pil Pack, Eun Hee Lee, Youngjoo Byun, Sang Eun Choi, Kenneth Sung Lee, Un Hwan Ha, Yong Woo Jung

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Androgen-disrupting chemicals (ADCs) can alter male sexual development. Although the effects of ADCs on hormone disruption have been studied, their influence on the immune response is not fully understood. To investigate the effects of ADCs on innate immunity, we tested eight candidate ADCs for their influence on macrophages by measuring nitric oxide (NO) production and cell viability. Our results showed that treatment with a mixture of lipopolysaccharide and hexachlorobenzene increased NO production in RAW 264.7 cells, a murine macrophage cell line. In contrast, compared to exposure to a negative control, exposure to di-2-ethylhexyl adipate (DEHA), benzylbutyl phthalate (BBP), testosterone (TTT), or permethrin decreased NO production. DEHA, BBP, and TTT inhibited NO production in an inducible nitric oxide synthase-dependent manner. Treatment with bisphenol A (BPA), nonylphenol (NNP), or tributyltin chloride (TBTC) reduced NO production and induced cell death. While BPA induced RAW 264.7 cell death through apoptosis, NNP and TBTC caused cell death through necrosis. These results offer insights into the influences of ADCs on the innate immune system.

Original language	English (US)
Pages (from-to)	649-656
Number of pages	8
Journal	Inflammation
Volume	37
Issue number	3
DOIs	https://doi.org/10.1007/s10753-013-9781-1
State	Published - 2014
Externally published	Yes

Keywords

androgen-disrupting chemicals
cell death
lipopolysaccharide
macrophage
nitric oxide

ASJC Scopus subject areas

Immunology
Immunology and Allergy
General Medicine

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10.1007/s10753-013-9781-1

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title = "Diverse influences of androgen-disrupting chemicals on immune responses mounted by macrophages",

abstract = "Androgen-disrupting chemicals (ADCs) can alter male sexual development. Although the effects of ADCs on hormone disruption have been studied, their influence on the immune response is not fully understood. To investigate the effects of ADCs on innate immunity, we tested eight candidate ADCs for their influence on macrophages by measuring nitric oxide (NO) production and cell viability. Our results showed that treatment with a mixture of lipopolysaccharide and hexachlorobenzene increased NO production in RAW 264.7 cells, a murine macrophage cell line. In contrast, compared to exposure to a negative control, exposure to di-2-ethylhexyl adipate (DEHA), benzylbutyl phthalate (BBP), testosterone (TTT), or permethrin decreased NO production. DEHA, BBP, and TTT inhibited NO production in an inducible nitric oxide synthase-dependent manner. Treatment with bisphenol A (BPA), nonylphenol (NNP), or tributyltin chloride (TBTC) reduced NO production and induced cell death. While BPA induced RAW 264.7 cell death through apoptosis, NNP and TBTC caused cell death through necrosis. These results offer insights into the influences of ADCs on the innate immune system.",

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author = "Kim, {Kyong Hoon} and Yeon, {Seung Min} and Kim, {Hyun Gyung} and Choi, {Hyun Suk} and Hyojeung Kang and Park, {Hee Deung} and Park, {Tae Won} and Pack, {Seung Pil} and Lee, {Eun Hee} and Youngjoo Byun and Choi, {Sang Eun} and Lee, {Kenneth Sung} and Ha, {Un Hwan} and Jung, {Yong Woo}",

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AU - Kim, Kyong Hoon

AU - Yeon, Seung Min

AU - Kim, Hyun Gyung

AU - Choi, Hyun Suk

AU - Kang, Hyojeung

AU - Park, Hee Deung

AU - Park, Tae Won

AU - Pack, Seung Pil

AU - Lee, Eun Hee

AU - Byun, Youngjoo

AU - Choi, Sang Eun

AU - Lee, Kenneth Sung

AU - Ha, Un Hwan

AU - Jung, Yong Woo

PY - 2014

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AB - Androgen-disrupting chemicals (ADCs) can alter male sexual development. Although the effects of ADCs on hormone disruption have been studied, their influence on the immune response is not fully understood. To investigate the effects of ADCs on innate immunity, we tested eight candidate ADCs for their influence on macrophages by measuring nitric oxide (NO) production and cell viability. Our results showed that treatment with a mixture of lipopolysaccharide and hexachlorobenzene increased NO production in RAW 264.7 cells, a murine macrophage cell line. In contrast, compared to exposure to a negative control, exposure to di-2-ethylhexyl adipate (DEHA), benzylbutyl phthalate (BBP), testosterone (TTT), or permethrin decreased NO production. DEHA, BBP, and TTT inhibited NO production in an inducible nitric oxide synthase-dependent manner. Treatment with bisphenol A (BPA), nonylphenol (NNP), or tributyltin chloride (TBTC) reduced NO production and induced cell death. While BPA induced RAW 264.7 cell death through apoptosis, NNP and TBTC caused cell death through necrosis. These results offer insights into the influences of ADCs on the innate immune system.

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Diverse influences of androgen-disrupting chemicals on immune responses mounted by macrophages

Abstract

Keywords

ASJC Scopus subject areas

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