Divergence of primate ribosomal RNA genes as assayed by restriction enzyme analysis

B. Nelkin; D. Strayer; B. Vogelstein

doi:10.1016/0378-1119(80)90089-X

Divergence of primate ribosomal RNA genes as assayed by restriction enzyme analysis

B. Nelkin, D. Strayer, B. Vogelstein

School of Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Primate ribosomal RNA (rRNA) genes have been compared by restriction endonuclease mapping. In all species examined, the restriction map of the reiterated ribosomal DNA is simple (within the limits of detection by hybridization with rRNA) and is consistent with a high degree of homogeneity among the repeats. Within a species, all members have similar rDNA restriction patterns. However, different species of primates have distinctly different rDNA restriction maps; even chimpanzee and man can be discerned by their rDNA restriction patterns. Possible mechanisms for maintenance of homogeneity of the rDNA repeats within a species, while allowing divergence among closely related species, are discussed.

Original language	English (US)
Pages (from-to)	89-96
Number of pages	8
Journal	Gene
Volume	11
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1119(80)90089-X
State	Published - 1980

Keywords

Phylogeny
RNA-DNA hybridization
SDS
kbp
kilobase pairs
multigene families
sodium dodecyl sulfate

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(80)90089-X

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title = "Divergence of primate ribosomal RNA genes as assayed by restriction enzyme analysis",

abstract = "Primate ribosomal RNA (rRNA) genes have been compared by restriction endonuclease mapping. In all species examined, the restriction map of the reiterated ribosomal DNA is simple (within the limits of detection by hybridization with rRNA) and is consistent with a high degree of homogeneity among the repeats. Within a species, all members have similar rDNA restriction patterns. However, different species of primates have distinctly different rDNA restriction maps; even chimpanzee and man can be discerned by their rDNA restriction patterns. Possible mechanisms for maintenance of homogeneity of the rDNA repeats within a species, while allowing divergence among closely related species, are discussed.",

keywords = "Phylogeny, RNA-DNA hybridization, SDS, kbp, kilobase pairs, multigene families, sodium dodecyl sulfate",

author = "B. Nelkin and D. Strayer and B. Vogelstein",

note = "Funding Information: This paper is in partial fulfillment of the requirements for the degree of Doctor of Philosophy, George Washington University (B.N.). We thank Dr. D.H. GiUespie for constant encouragement and advice, Dr. R.C. GaUo for providing support and facilities for this research and Dr. Wolf Prensky for providing the \[12SI\]rDNA. This work was partially supported by NIH grant No. CA 06973 and a gift from Bristol Myers Company.",

year = "1980",

doi = "10.1016/0378-1119(80)90089-X",

language = "English (US)",

volume = "11",

pages = "89--96",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1-2",

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T1 - Divergence of primate ribosomal RNA genes as assayed by restriction enzyme analysis

AU - Nelkin, B.

AU - Strayer, D.

AU - Vogelstein, B.

N1 - Funding Information: This paper is in partial fulfillment of the requirements for the degree of Doctor of Philosophy, George Washington University (B.N.). We thank Dr. D.H. GiUespie for constant encouragement and advice, Dr. R.C. GaUo for providing support and facilities for this research and Dr. Wolf Prensky for providing the \[12SI\]rDNA. This work was partially supported by NIH grant No. CA 06973 and a gift from Bristol Myers Company.

PY - 1980

Y1 - 1980

N2 - Primate ribosomal RNA (rRNA) genes have been compared by restriction endonuclease mapping. In all species examined, the restriction map of the reiterated ribosomal DNA is simple (within the limits of detection by hybridization with rRNA) and is consistent with a high degree of homogeneity among the repeats. Within a species, all members have similar rDNA restriction patterns. However, different species of primates have distinctly different rDNA restriction maps; even chimpanzee and man can be discerned by their rDNA restriction patterns. Possible mechanisms for maintenance of homogeneity of the rDNA repeats within a species, while allowing divergence among closely related species, are discussed.

AB - Primate ribosomal RNA (rRNA) genes have been compared by restriction endonuclease mapping. In all species examined, the restriction map of the reiterated ribosomal DNA is simple (within the limits of detection by hybridization with rRNA) and is consistent with a high degree of homogeneity among the repeats. Within a species, all members have similar rDNA restriction patterns. However, different species of primates have distinctly different rDNA restriction maps; even chimpanzee and man can be discerned by their rDNA restriction patterns. Possible mechanisms for maintenance of homogeneity of the rDNA repeats within a species, while allowing divergence among closely related species, are discussed.

KW - Phylogeny

KW - RNA-DNA hybridization

KW - SDS

KW - kbp

KW - kilobase pairs

KW - multigene families

KW - sodium dodecyl sulfate

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U2 - 10.1016/0378-1119(80)90089-X

DO - 10.1016/0378-1119(80)90089-X

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AN - SCOPUS:0019202433

SN - 0378-1119

VL - 11

SP - 89

EP - 96

JO - Gene

JF - Gene

IS - 1-2

ER -

Divergence of primate ribosomal RNA genes as assayed by restriction enzyme analysis

Abstract

Keywords

ASJC Scopus subject areas

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