Distinct mechanism of human neuroblastoma cell adhesion to fibronectin

T. Yoshihara, S. Ikushima, Y. Shimizu, N. Esumi, S. Todo, M. J. Humphries, S. Imashuku

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


We investigated the adhesion of three morphologically distinct human neuroblastoma cell lines (NCG, GOTO and SK-N-DZ) to intact fibronectin, central cell binding domain fragment (CBF) and CS peptide-IgG conjugates in the fibronectin molecule. Each cell line was found to express different integrin fibronectin receptors (α3β1, α4β1 and α5β1), although similarly attached on intact fibronectin. To CBF, NCG attached well, while GOTO moderately and SK-N-DZ poorly attached. Only GOTO adhered to CS1-IgG. RGDS inhibited the spreading of NCG and SK-N-DZ on intact fibronectin, but it barely inhibited that of GOTO. The analysis by fluorescence-activated cell sorting (FACS) revealed that NCG expressed abundant α3β1 and α5β1, but little α4β1, while GOTO expressed a large amount of α4β1 as well as α5β1. SK-N-DZ was undetectable in any of these molecules, but expressed αvβ1, which was identified by immunoprecipitation and immunoblotting. Polyclonal antibody to αvβ3 inhibited the adhesion of SK-N-DZ but not that of NCG or GOTO on intact fibronectin. These results suggest the existence of a distinct mechanism of cell adhesion to fibronectin among human neuroblastoma cell lines. It remains to be determined if such heterogeneous adhesion properties are related to the unique metastatic character of human neuroblastoma.

Original languageEnglish (US)
Pages (from-to)363-375
Number of pages13
JournalClinical & Experimental Metastasis
Issue number4
StatePublished - Jul 1991
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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