Disruption of the Pfg27 locus by homologous recombination leads to loss of the sexual phenotype in P. falciparum

Cheryl Ann Lobo; Hisashi Fujioka; Masamichi Aikawa; Nirbhay Kumar

doi:10.1016/S1097-2765(01)80011-3

Disruption of the Pfg27 locus by homologous recombination leads to loss of the sexual phenotype in P. falciparum

Cheryl Ann Lobo, Hisashi Fujioka, Masamichi Aikawa, Nirbhay Kumar

Research output: Contribution to journal › Article › peer-review

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Abstract

Transmission of malaria depends upon the differentiation and development of the sexual stages of the parasite. In Plasmodium falciparum, it is a complex, multistage process, involving the expression of a large number of sexual stage-specific proteins. Pfg27 is one such protein, abundantly expressed at the onset of gametocytogenesis. We report successful disruption of the Pfg27 locus using homologous recombination and show that it is essential for the maintenance of the sexual phenotype. Transfectants lacking Pfg27 abort early in sexual development, resulting in vacuolated, highly disarranged, and disintegrating parasites. This suggests a critical role for Pfg27 in the sexual development of the parasite.

Original language	English (US)
Pages (from-to)	793-798
Number of pages	6
Journal	Molecular cell
Volume	3
Issue number	6
DOIs	https://doi.org/10.1016/S1097-2765(01)80011-3
State	Published - Jun 1999
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/S1097-2765(01)80011-3

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@article{154460d378da4d6c886595cf55b9e3db,

title = "Disruption of the Pfg27 locus by homologous recombination leads to loss of the sexual phenotype in P. falciparum",

abstract = "Transmission of malaria depends upon the differentiation and development of the sexual stages of the parasite. In Plasmodium falciparum, it is a complex, multistage process, involving the expression of a large number of sexual stage-specific proteins. Pfg27 is one such protein, abundantly expressed at the onset of gametocytogenesis. We report successful disruption of the Pfg27 locus using homologous recombination and show that it is essential for the maintenance of the sexual phenotype. Transfectants lacking Pfg27 abort early in sexual development, resulting in vacuolated, highly disarranged, and disintegrating parasites. This suggests a critical role for Pfg27 in the sexual development of the parasite.",

author = "Lobo, {Cheryl Ann} and Hisashi Fujioka and Masamichi Aikawa and Nirbhay Kumar",

note = "Funding Information: This work was supported by grants from the NIH, AI 40089 (N. K.), and AI35827 (M. A. and H. F.). We thank Yimin Wu and Kirk Deitsch for helpful discussions and for providing us with the pDT-Tg23 vector, David Fidock for providing us with some of the primers, Clive Shiff for use of the immunofluorescence microscope, and Paul Englund for use of the PFGE apparatus.",

year = "1999",

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doi = "10.1016/S1097-2765(01)80011-3",

language = "English (US)",

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pages = "793--798",

journal = "Molecular Cell",

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T1 - Disruption of the Pfg27 locus by homologous recombination leads to loss of the sexual phenotype in P. falciparum

AU - Lobo, Cheryl Ann

AU - Fujioka, Hisashi

AU - Aikawa, Masamichi

AU - Kumar, Nirbhay

N1 - Funding Information: This work was supported by grants from the NIH, AI 40089 (N. K.), and AI35827 (M. A. and H. F.). We thank Yimin Wu and Kirk Deitsch for helpful discussions and for providing us with the pDT-Tg23 vector, David Fidock for providing us with some of the primers, Clive Shiff for use of the immunofluorescence microscope, and Paul Englund for use of the PFGE apparatus.

PY - 1999/6

Y1 - 1999/6

N2 - Transmission of malaria depends upon the differentiation and development of the sexual stages of the parasite. In Plasmodium falciparum, it is a complex, multistage process, involving the expression of a large number of sexual stage-specific proteins. Pfg27 is one such protein, abundantly expressed at the onset of gametocytogenesis. We report successful disruption of the Pfg27 locus using homologous recombination and show that it is essential for the maintenance of the sexual phenotype. Transfectants lacking Pfg27 abort early in sexual development, resulting in vacuolated, highly disarranged, and disintegrating parasites. This suggests a critical role for Pfg27 in the sexual development of the parasite.

AB - Transmission of malaria depends upon the differentiation and development of the sexual stages of the parasite. In Plasmodium falciparum, it is a complex, multistage process, involving the expression of a large number of sexual stage-specific proteins. Pfg27 is one such protein, abundantly expressed at the onset of gametocytogenesis. We report successful disruption of the Pfg27 locus using homologous recombination and show that it is essential for the maintenance of the sexual phenotype. Transfectants lacking Pfg27 abort early in sexual development, resulting in vacuolated, highly disarranged, and disintegrating parasites. This suggests a critical role for Pfg27 in the sexual development of the parasite.

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JO - Molecular Cell

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Disruption of the Pfg27 locus by homologous recombination leads to loss of the sexual phenotype in P. falciparum

Abstract

ASJC Scopus subject areas

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