TY - CHAP
T1 - Discovery and Quantitative Dissection of Cytokinesis Mechanisms Using Dictyostelium discoideum
AU - Nguyen, Jennifer M.K.
AU - Liu, Yinan
AU - Nguyen, Ly
AU - Sidhaye, Venkataramana K.
AU - Robinson, Douglas N.
N1 - Publisher Copyright:
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
PY - 2024
Y1 - 2024
N2 - The social amoeba Dictyostelium discoideum is a versatile model for understanding many different cellular processes involving cell motility including chemotaxis, phagocytosis, and cytokinesis. Cytokinesis, in particular, is a model cell-shaped change process in which a cell separates into two daughter cells. D. discoideum has been used extensively to identify players in cytokinesis and understand how they comprise the mechanosensory and biochemical pathways of cytokinesis. In this chapter, we describe how we use cDNA library complementation with D. discoideum to discover potential regulators of cytokinesis. Once identified, these regulators are further analyzed through live cell imaging, immunofluorescence imaging, fluorescence correlation and cross-correlation spectroscopy, micropipette aspiration, and fluorescence recovery after photobleaching. Collectively, these methods aid in detailing the mechanisms and signaling pathways that comprise cell division.
AB - The social amoeba Dictyostelium discoideum is a versatile model for understanding many different cellular processes involving cell motility including chemotaxis, phagocytosis, and cytokinesis. Cytokinesis, in particular, is a model cell-shaped change process in which a cell separates into two daughter cells. D. discoideum has been used extensively to identify players in cytokinesis and understand how they comprise the mechanosensory and biochemical pathways of cytokinesis. In this chapter, we describe how we use cDNA library complementation with D. discoideum to discover potential regulators of cytokinesis. Once identified, these regulators are further analyzed through live cell imaging, immunofluorescence imaging, fluorescence correlation and cross-correlation spectroscopy, micropipette aspiration, and fluorescence recovery after photobleaching. Collectively, these methods aid in detailing the mechanisms and signaling pathways that comprise cell division.
KW - Cytoskeletal network
KW - Dictyostelium discoideum
KW - Mechanoresponsive
KW - Protein dynamics
KW - cDNA library complementation
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U2 - 10.1007/978-1-0716-3894-1_1
DO - 10.1007/978-1-0716-3894-1_1
M3 - Chapter
C2 - 38954194
AN - SCOPUS:85197709316
T3 - Methods in Molecular Biology
SP - 1
EP - 27
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -