Discordance between changes in the gut microbiota and pathogenicity in a mouse model of spontaneous colitis

Maria Elisa Perez-Muñoz; Kirk Bergstrom; Vincent Peng; Robert Schmaltz; Roberto Jimenez-Cardona; Nathan Marsteller; Sam McGee; Thomas Clavel; Ruth Ley; Jianxin Fu; Lijun Xia; Daniel A. Peterson

doi:10.4161/gmic.28622

Discordance between changes in the gut microbiota and pathogenicity in a mouse model of spontaneous colitis

Maria Elisa Perez-Muñoz, Kirk Bergstrom, Vincent Peng, Robert Schmaltz, Roberto Jimenez-Cardona, Nathan Marsteller, Sam McGee, Thomas Clavel, Ruth Ley, Jianxin Fu, Lijun Xia, Daniel A. Peterson

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Under conventional conditions, mice deficient in core 1-derived O-glycans (TM-IEC C1galt1^-/-), which have a defective mucus layer, experienced spontaneous inflammation of the colon. Analysis of fecal bacterial populations by pyrosequencing of 16S rRNA gene showed that disease in conventional TM-IEC C1galt1^-/- was associated with shifts in the microbiota manifested by increases in Lactobacillus and Clostridium species, and decreases in unclassified Ruminococcaceae and Lachnospiraceae. Under germ-free (GF) conditions, TM-IEC C1galt1^-/- presented decreased goblet cells, but did not develop inflammation. Monoassociation of GF TM-IEC C1galt1^-/- revealed that bacterial species differ significantly in their ability to induce inflammatory changes. Bacteroides thetaiotaomicron caused inflammation, while Lactobacillus johnsonii (enriched during colitis) did not. These observations demonstrate that not all microbiota shifts that correlate with disease contribute to pathogenesis.

Original language	English (US)
Journal	Gut Microbes
Volume	5
Issue number	3
DOIs	https://doi.org/10.4161/gmic.28622
State	Published - Mar 24 2014
Externally published	Yes

Keywords

Akkermasia muciniphila
Bacteroides sartorii
Bacteroides thetaiotaomicron
Clostridium
Core 1 O-glycans deficient mice
Dysbiosis
Gut microbiota
Lactobacillus johnsonii
Pyrosequencing
Spontaneous colitis

ASJC Scopus subject areas

Microbiology
Gastroenterology
Microbiology (medical)
Infectious Diseases

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10.4161/gmic.28622

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title = "Discordance between changes in the gut microbiota and pathogenicity in a mouse model of spontaneous colitis",

abstract = "Under conventional conditions, mice deficient in core 1-derived O-glycans (TM-IEC C1galt1-/-), which have a defective mucus layer, experienced spontaneous inflammation of the colon. Analysis of fecal bacterial populations by pyrosequencing of 16S rRNA gene showed that disease in conventional TM-IEC C1galt1-/- was associated with shifts in the microbiota manifested by increases in Lactobacillus and Clostridium species, and decreases in unclassified Ruminococcaceae and Lachnospiraceae. Under germ-free (GF) conditions, TM-IEC C1galt1-/- presented decreased goblet cells, but did not develop inflammation. Monoassociation of GF TM-IEC C1galt1-/- revealed that bacterial species differ significantly in their ability to induce inflammatory changes. Bacteroides thetaiotaomicron caused inflammation, while Lactobacillus johnsonii (enriched during colitis) did not. These observations demonstrate that not all microbiota shifts that correlate with disease contribute to pathogenesis.",

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AU - Perez-Muñoz, Maria Elisa

AU - Bergstrom, Kirk

AU - Peng, Vincent

AU - Schmaltz, Robert

AU - Jimenez-Cardona, Roberto

AU - Marsteller, Nathan

AU - McGee, Sam

AU - Clavel, Thomas

AU - Ley, Ruth

AU - Fu, Jianxin

AU - Xia, Lijun

AU - Peterson, Daniel A.

PY - 2014/3/24

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AB - Under conventional conditions, mice deficient in core 1-derived O-glycans (TM-IEC C1galt1-/-), which have a defective mucus layer, experienced spontaneous inflammation of the colon. Analysis of fecal bacterial populations by pyrosequencing of 16S rRNA gene showed that disease in conventional TM-IEC C1galt1-/- was associated with shifts in the microbiota manifested by increases in Lactobacillus and Clostridium species, and decreases in unclassified Ruminococcaceae and Lachnospiraceae. Under germ-free (GF) conditions, TM-IEC C1galt1-/- presented decreased goblet cells, but did not develop inflammation. Monoassociation of GF TM-IEC C1galt1-/- revealed that bacterial species differ significantly in their ability to induce inflammatory changes. Bacteroides thetaiotaomicron caused inflammation, while Lactobacillus johnsonii (enriched during colitis) did not. These observations demonstrate that not all microbiota shifts that correlate with disease contribute to pathogenesis.

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KW - Pyrosequencing

KW - Spontaneous colitis

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Discordance between changes in the gut microbiota and pathogenicity in a mouse model of spontaneous colitis

Abstract

Keywords

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