Dirofilaria immitis: Identification and characterization of circulating parasite antigens

John P. Ehrenberg; Wesley K. Tamashiro; Alan L. Scott

doi:10.1016/0014-4894(87)90163-9

Dirofilaria immitis: Identification and characterization of circulating parasite antigens

John P. Ehrenberg, Wesley K. Tamashiro, Alan L. Scott

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A method for the identification of circulating parasite antigens in filarial nematode infections was developed using canine infections with Dirofilaria immitis as a model. Filarial antigens ranging in molecular weight from 211 to 13 kDa were extracted from the sera of microfilaremic dogs by a solid phase immunobinding procedure and identified by immunostaining of Western blots. A major antigen of 104 kDa was selected for further characterization. The 104 kDa circulating antigen showed antigenic and biochemical identity with 104 kDa peptides found in extracts of adult male and microfilarial stages of the parasite. The 104 kDa peptide was antigenically stable under a variety of storage conditions. Its potential as a diagnostic target is discussed.

Original language	English (US)
Pages (from-to)	205-214
Number of pages	10
Journal	Experimental Parasitology
Volume	63
Issue number	2
DOIs	https://doi.org/10.1016/0014-4894(87)90163-9
State	Published - Apr 1987

Keywords

Antigen detection assay
Circulating antigen
Cross reacting antigens
Dirofilaria immitis
Dog
Filarioidea
Immune complexed antigen
Nematode, parasitic

ASJC Scopus subject areas

Parasitology
Immunology
Infectious Diseases

Access to Document

10.1016/0014-4894(87)90163-9

Cite this

@article{e3c1c83e687a493090acc25cb2cf12ac,

title = "Dirofilaria immitis: Identification and characterization of circulating parasite antigens",

abstract = "A method for the identification of circulating parasite antigens in filarial nematode infections was developed using canine infections with Dirofilaria immitis as a model. Filarial antigens ranging in molecular weight from 211 to 13 kDa were extracted from the sera of microfilaremic dogs by a solid phase immunobinding procedure and identified by immunostaining of Western blots. A major antigen of 104 kDa was selected for further characterization. The 104 kDa circulating antigen showed antigenic and biochemical identity with 104 kDa peptides found in extracts of adult male and microfilarial stages of the parasite. The 104 kDa peptide was antigenically stable under a variety of storage conditions. Its potential as a diagnostic target is discussed.",

keywords = "Antigen detection assay, Circulating antigen, Cross reacting antigens, Dirofilaria immitis, Dog, Filarioidea, Immune complexed antigen, Nematode, parasitic",

author = "Ehrenberg, {John P.} and Tamashiro, {Wesley K.} and Scott, {Alan L.}",

note = "Funding Information: John P. Ehrenbergw as supportedb y Grant T16/181/ M8/E.26 from the World Health Organization.W esley K. Tamashiro was supported by Training Grant T32 A107057f rom the U.S. National Instituteso f Health. We thank Mr. ChamberlainD iala for expert technical assistancea nd Dr. Robert Hamilton for his valuable commentsa nd suggestions.",

year = "1987",

month = apr,

doi = "10.1016/0014-4894(87)90163-9",

language = "English (US)",

volume = "63",

pages = "205--214",

journal = "Experimental Parasitology",

issn = "0014-4894",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Dirofilaria immitis

T2 - Identification and characterization of circulating parasite antigens

AU - Ehrenberg, John P.

AU - Tamashiro, Wesley K.

AU - Scott, Alan L.

N1 - Funding Information: John P. Ehrenbergw as supportedb y Grant T16/181/ M8/E.26 from the World Health Organization.W esley K. Tamashiro was supported by Training Grant T32 A107057f rom the U.S. National Instituteso f Health. We thank Mr. ChamberlainD iala for expert technical assistancea nd Dr. Robert Hamilton for his valuable commentsa nd suggestions.

PY - 1987/4

Y1 - 1987/4

N2 - A method for the identification of circulating parasite antigens in filarial nematode infections was developed using canine infections with Dirofilaria immitis as a model. Filarial antigens ranging in molecular weight from 211 to 13 kDa were extracted from the sera of microfilaremic dogs by a solid phase immunobinding procedure and identified by immunostaining of Western blots. A major antigen of 104 kDa was selected for further characterization. The 104 kDa circulating antigen showed antigenic and biochemical identity with 104 kDa peptides found in extracts of adult male and microfilarial stages of the parasite. The 104 kDa peptide was antigenically stable under a variety of storage conditions. Its potential as a diagnostic target is discussed.

AB - A method for the identification of circulating parasite antigens in filarial nematode infections was developed using canine infections with Dirofilaria immitis as a model. Filarial antigens ranging in molecular weight from 211 to 13 kDa were extracted from the sera of microfilaremic dogs by a solid phase immunobinding procedure and identified by immunostaining of Western blots. A major antigen of 104 kDa was selected for further characterization. The 104 kDa circulating antigen showed antigenic and biochemical identity with 104 kDa peptides found in extracts of adult male and microfilarial stages of the parasite. The 104 kDa peptide was antigenically stable under a variety of storage conditions. Its potential as a diagnostic target is discussed.

KW - Antigen detection assay

KW - Circulating antigen

KW - Cross reacting antigens

KW - Dirofilaria immitis

KW - Dog

KW - Filarioidea

KW - Immune complexed antigen

KW - Nematode, parasitic

UR - http://www.scopus.com/inward/record.url?scp=0023221858&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023221858&partnerID=8YFLogxK

U2 - 10.1016/0014-4894(87)90163-9

DO - 10.1016/0014-4894(87)90163-9

M3 - Article

C2 - 3552713

AN - SCOPUS:0023221858

SN - 0014-4894

VL - 63

SP - 205

EP - 214

JO - Experimental Parasitology

JF - Experimental Parasitology

IS - 2

ER -

Dirofilaria immitis: Identification and characterization of circulating parasite antigens

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this