Abstract
A method for the identification of circulating parasite antigens in filarial nematode infections was developed using canine infections with Dirofilaria immitis as a model. Filarial antigens ranging in molecular weight from 211 to 13 kDa were extracted from the sera of microfilaremic dogs by a solid phase immunobinding procedure and identified by immunostaining of Western blots. A major antigen of 104 kDa was selected for further characterization. The 104 kDa circulating antigen showed antigenic and biochemical identity with 104 kDa peptides found in extracts of adult male and microfilarial stages of the parasite. The 104 kDa peptide was antigenically stable under a variety of storage conditions. Its potential as a diagnostic target is discussed.
Original language | English (US) |
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Pages (from-to) | 205-214 |
Number of pages | 10 |
Journal | Experimental Parasitology |
Volume | 63 |
Issue number | 2 |
DOIs | |
State | Published - Apr 1987 |
Keywords
- Antigen detection assay
- Circulating antigen
- Cross reacting antigens
- Dirofilaria immitis
- Dog
- Filarioidea
- Immune complexed antigen
- Nematode, parasitic
ASJC Scopus subject areas
- Parasitology
- Immunology
- Infectious Diseases