Direct regulation of GnRH transcription by CRF-like peptides in an immortalized neuronal cell line

D. J. Tellam; M. J. Perone; I. C. Dunn; S. Radovick; J. Brennand; J. E. Rivier; M. G. Castro; D. A. Lovejoy

Direct regulation of GnRH transcription by CRF-like peptides in an immortalized neuronal cell line

D. J. Tellam, M. J. Perone, I. C. Dunn, S. Radovick, J. Brennand, J. E. Rivier, M. G. Castro, D. A. Lovejoy

Research output: Contribution to journal › Article › peer-review

Abstract

The existence of a CRF-dependent inhibition of GnRH transcription was investigated using a neuronal GnRH-expressing cell line (Gn11) stably transfected with mouse (-611 bp) or chicken (-3000 bp) GnRH promoter/luciferase reporter constructs. The presence of the CRF-R1 receptor was established using a specific CRF-R1 antiserum. After 7 h of incubation, urotensin-I and sauvagine increased the mouse GnRH-reporter bioluminescence by 1.3- and 1.2-fold, respectively, compared with control cells. Subsequently, CRF, urotensin-I and sauvagine decreased luciferase reporter activity to about 60% of the control values after 14 h. Similar trends occurred with the chicken GnRH promoter with UI increasing reporter gene activity 2.4-fold over the controls after 14 h incubation. These data provide additional evidence for the direct regulation of GnRH transcription by CRF- like peptides.

Original language	English (US)
Pages (from-to)	3135-3140
Number of pages	6
Journal	NeuroReport
Volume	9
Issue number	14
State	Published - Oct 5 1998
Externally published	Yes

Keywords

Reproduction
Sauvagine
Stress
Transcription
Urotensin-I

ASJC Scopus subject areas

Neuroscience(all)

Cite this

@article{61f904507d31464aa3ed109b4a24bd40,

title = "Direct regulation of GnRH transcription by CRF-like peptides in an immortalized neuronal cell line",

abstract = "The existence of a CRF-dependent inhibition of GnRH transcription was investigated using a neuronal GnRH-expressing cell line (Gn11) stably transfected with mouse (-611 bp) or chicken (-3000 bp) GnRH promoter/luciferase reporter constructs. The presence of the CRF-R1 receptor was established using a specific CRF-R1 antiserum. After 7 h of incubation, urotensin-I and sauvagine increased the mouse GnRH-reporter bioluminescence by 1.3- and 1.2-fold, respectively, compared with control cells. Subsequently, CRF, urotensin-I and sauvagine decreased luciferase reporter activity to about 60% of the control values after 14 h. Similar trends occurred with the chicken GnRH promoter with UI increasing reporter gene activity 2.4-fold over the controls after 14 h incubation. These data provide additional evidence for the direct regulation of GnRH transcription by CRF- like peptides.",

keywords = "Reproduction, Sauvagine, Stress, Transcription, Urotensin-I",

author = "Tellam, {D. J.} and Perone, {M. J.} and Dunn, {I. C.} and S. Radovick and J. Brennand and Rivier, {J. E.} and Castro, {M. G.} and Lovejoy, {D. A.}",

year = "1998",

month = oct,

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language = "English (US)",

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T1 - Direct regulation of GnRH transcription by CRF-like peptides in an immortalized neuronal cell line

AU - Tellam, D. J.

AU - Perone, M. J.

AU - Dunn, I. C.

AU - Radovick, S.

AU - Brennand, J.

AU - Rivier, J. E.

AU - Castro, M. G.

AU - Lovejoy, D. A.

PY - 1998/10/5

Y1 - 1998/10/5

N2 - The existence of a CRF-dependent inhibition of GnRH transcription was investigated using a neuronal GnRH-expressing cell line (Gn11) stably transfected with mouse (-611 bp) or chicken (-3000 bp) GnRH promoter/luciferase reporter constructs. The presence of the CRF-R1 receptor was established using a specific CRF-R1 antiserum. After 7 h of incubation, urotensin-I and sauvagine increased the mouse GnRH-reporter bioluminescence by 1.3- and 1.2-fold, respectively, compared with control cells. Subsequently, CRF, urotensin-I and sauvagine decreased luciferase reporter activity to about 60% of the control values after 14 h. Similar trends occurred with the chicken GnRH promoter with UI increasing reporter gene activity 2.4-fold over the controls after 14 h incubation. These data provide additional evidence for the direct regulation of GnRH transcription by CRF- like peptides.

AB - The existence of a CRF-dependent inhibition of GnRH transcription was investigated using a neuronal GnRH-expressing cell line (Gn11) stably transfected with mouse (-611 bp) or chicken (-3000 bp) GnRH promoter/luciferase reporter constructs. The presence of the CRF-R1 receptor was established using a specific CRF-R1 antiserum. After 7 h of incubation, urotensin-I and sauvagine increased the mouse GnRH-reporter bioluminescence by 1.3- and 1.2-fold, respectively, compared with control cells. Subsequently, CRF, urotensin-I and sauvagine decreased luciferase reporter activity to about 60% of the control values after 14 h. Similar trends occurred with the chicken GnRH promoter with UI increasing reporter gene activity 2.4-fold over the controls after 14 h incubation. These data provide additional evidence for the direct regulation of GnRH transcription by CRF- like peptides.

KW - Reproduction

KW - Sauvagine

KW - Stress

KW - Transcription

KW - Urotensin-I

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AN - SCOPUS:0032487508

SN - 0959-4965

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JF - NeuroReport

IS - 14

ER -

Direct regulation of GnRH transcription by CRF-like peptides in an immortalized neuronal cell line

Abstract

Keywords

ASJC Scopus subject areas

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