Abstract
The existence of a CRF-dependent inhibition of GnRH transcription was investigated using a neuronal GnRH-expressing cell line (Gn11) stably transfected with mouse (-611 bp) or chicken (-3000 bp) GnRH promoter/luciferase reporter constructs. The presence of the CRF-R1 receptor was established using a specific CRF-R1 antiserum. After 7 h of incubation, urotensin-I and sauvagine increased the mouse GnRH-reporter bioluminescence by 1.3- and 1.2-fold, respectively, compared with control cells. Subsequently, CRF, urotensin-I and sauvagine decreased luciferase reporter activity to about 60% of the control values after 14 h. Similar trends occurred with the chicken GnRH promoter with UI increasing reporter gene activity 2.4-fold over the controls after 14 h incubation. These data provide additional evidence for the direct regulation of GnRH transcription by CRF- like peptides.
Original language | English (US) |
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Pages (from-to) | 3135-3140 |
Number of pages | 6 |
Journal | NeuroReport |
Volume | 9 |
Issue number | 14 |
State | Published - Oct 5 1998 |
Externally published | Yes |
Keywords
- Reproduction
- Sauvagine
- Stress
- Transcription
- Urotensin-I
ASJC Scopus subject areas
- Neuroscience(all)