Direct detection and quantification of neoantigens

Qing Wang, Jacqueline Douglass, Michael S. Hwang, Emily Han Chung Hsiue, Brian J. Mog, Ming Zhang, Nickolas Papadopoulos, Kenneth W. Kinzler, Shibin Zhou, Bert Vogelstein

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Many immunotherapeutic approaches under development rely on T-cell recognition of cancer-derived peptides bound to human leukocyte antigen molecules on the cell surface. Direct experimental demonstration that such peptides are processed and bound is currently challenging. Here, we describe a method that meets this challenge. The method entailed an optimized immunoprecipitation protocol coupled with twodimensional chromatography and mass spectrometry. The ability to detect and quantify minute amounts of predefined antigens should be useful both for basic research in tumor immunology and for the development of rationally designed cancer vaccines.

Original languageEnglish (US)
Pages (from-to)1748-1754
Number of pages7
JournalCancer Immunology Research
Issue number11
StatePublished - 2019

ASJC Scopus subject areas

  • Immunology
  • Cancer Research


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