Digital DNA Sequence Profiling of Rare Epigenetic Cancer Biomarkers in A Highly Parallelized Microfluidic Platform

Christine M. O'Keefe; Sixuan Li; Tza Huei Jeff Wang

doi:10.1109/TRANSDUCERS.2019.8808784

Digital DNA Sequence Profiling of Rare Epigenetic Cancer Biomarkers in A Highly Parallelized Microfluidic Platform

Christine M. O'Keefe
, Sixuan Li
, Tza Huei Jeff Wang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We present a microfluidic platform for molecule-by-molecule detection of heterogeneous epigenetic patterns of rare tumor-derived DNA by highly parallelized digital melt assessment. We introduce a multilayer geometric strategy to capture single molecules in a pico-array at high efficiency. We demonstrate sensitive detection and comprehensive profiling of rare heterogeneously methylated DNA of a classic tumor suppressor gene, p14^ARF, at frequencies as low as 0.0001% methylated/unmethylated.

Original language	English (US)
Title of host publication	2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	2-5
Number of pages	4
ISBN (Electronic)	9781728120072
DOIs	https://doi.org/10.1109/TRANSDUCERS.2019.8808784
State	Published - Jun 2019
Externally published	Yes
Event	20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany Duration: Jun 23 2019 → Jun 27 2019

Publication series

Name	2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference	20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
Country/Territory	Germany
City	Berlin
Period	6/23/19 → 6/27/19

Keywords

Microfluidic DNA capture efficiency
circulating DNA
digital melt
heterogeneity
molecule-by-molecule profiling

ASJC Scopus subject areas

Process Chemistry and Technology
Spectroscopy
Electrical and Electronic Engineering
Mechanical Engineering
Electronic, Optical and Magnetic Materials
Control and Optimization
Instrumentation

Access to Document

10.1109/TRANSDUCERS.2019.8808784

Cite this

O'Keefe, C. M., Li, S., & Wang, T. H. J. (2019). Digital DNA Sequence Profiling of Rare Epigenetic Cancer Biomarkers in A Highly Parallelized Microfluidic Platform. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (pp. 2-5). Article 8808784 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808784

Digital DNA Sequence Profiling of Rare Epigenetic Cancer Biomarkers in A Highly Parallelized Microfluidic Platform. / O'Keefe, Christine M.; Li, Sixuan; Wang, Tza Huei Jeff.
2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. p. 2-5 8808784 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

O'Keefe, CM, Li, S & Wang, THJ 2019, Digital DNA Sequence Profiling of Rare Epigenetic Cancer Biomarkers in A Highly Parallelized Microfluidic Platform. in 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII., 8808784, 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Institute of Electrical and Electronics Engineers Inc., pp. 2-5, 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Berlin, Germany, 6/23/19. https://doi.org/10.1109/TRANSDUCERS.2019.8808784

O'Keefe CM, Li S, Wang THJ. Digital DNA Sequence Profiling of Rare Epigenetic Cancer Biomarkers in A Highly Parallelized Microfluidic Platform. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc. 2019. p. 2-5. 8808784. (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). doi: 10.1109/TRANSDUCERS.2019.8808784

O'Keefe, Christine M. ; Li, Sixuan ; Wang, Tza Huei Jeff. / Digital DNA Sequence Profiling of Rare Epigenetic Cancer Biomarkers in A Highly Parallelized Microfluidic Platform. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 2-5 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

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abstract = "We present a microfluidic platform for molecule-by-molecule detection of heterogeneous epigenetic patterns of rare tumor-derived DNA by highly parallelized digital melt assessment. We introduce a multilayer geometric strategy to capture single molecules in a pico-array at high efficiency. We demonstrate sensitive detection and comprehensive profiling of rare heterogeneously methylated DNA of a classic tumor suppressor gene, p14ARF, at frequencies as low as 0.0001\% methylated/unmethylated.",

keywords = "Microfluidic DNA capture efficiency, circulating DNA, digital melt, heterogeneity, molecule-by-molecule profiling",

author = "O'Keefe, \{Christine M.\} and Sixuan Li and Wang, \{Tza Huei Jeff\}",

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Digital DNA Sequence Profiling of Rare Epigenetic Cancer Biomarkers in A Highly Parallelized Microfluidic Platform

Abstract

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Keywords

ASJC Scopus subject areas

Access to Document

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