Diffusional mobility of Golgi proteins in membranes of living cells

Nelson B. Cole, Carolyn L. Smith, Noah Sciaky, Mark Terasaki, Michael Edidin, Jennifer Lippincott-Schwartz

Research output: Contribution to journalArticlepeer-review

393 Scopus citations


The mechanism by which Golgi membrane proteins are retained within the Golgi complex in the midst of a continuous flow of protein and lipid is not yet understood. The diffusional mobilities of mammalian Golgi membrane proteins fused with green fluorescent protein from Aequorea victoria were measured in living HeLa cells with the fluorescence photobleaching recovery technique. The diffusion coefficients ranged from 3 X 10-9 square centimeters per second to 5 x 10-9 square centimeters per second, with greater than 90 percent of the chimeric proteins mobile. Extensive lateral diffusion of the chimeric proteins occurred between Golgi stacks. Thus, the chimeras diffuse rapidly and freely in Golgi membranes, which suggests that Golgi targeting and retention of these molecules does not depend on protein immobilization.

Original languageEnglish (US)
Pages (from-to)797-801
Number of pages5
Issue number5276
StatePublished - 1996

ASJC Scopus subject areas

  • General


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