Abstract
Kawashiro, Nüsse & Scheid (1975) proposed a "diffusion method" of measurement of the metabolic rate in slices of tissue, and derived a mathematical relationship between the parameters which could be measured experimentally without penetration into the tissue, and the metabolic rate; the latter was assumed to obey zero-order chemical kinetics. In the present paper, we present a mathematical description of the steady-state diffusion in tissue slices with metabolism obeying an uptake kinetics of the Michaelis-Menten type, and demonstrate that the "diffusion method" is suitable for determination of the parameters of the Michaelis-Menten kinetics. It is analyzed how to choose the thickness of the tissue slice in order to achieve the maximum resolution of the method.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 325-332 |
| Number of pages | 8 |
| Journal | Journal of Theoretical Biology |
| Volume | 80 |
| Issue number | 3 |
| DOIs | |
| State | Published - Oct 7 1979 |
| Externally published | Yes |
ASJC Scopus subject areas
- Statistics and Probability
- Modeling and Simulation
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
- Agricultural and Biological Sciences(all)
- Applied Mathematics