@inbook{5f48579e9bc141e5b7c651971f45bbb0,
title = "Differentiation of adipocytes in monolayer from mouse embryonic stem cells",
abstract = "Obesity and its comorbidity incidence have increased worldwide during the past 10 years. In consequence, researchers have drawn their attention to the understanding of adipocyte differentiation. Several cellular model systems have been established; however no efficient protocol could be developed so far to differentiate the pluripotent embryonic stem cells to adipocytes. In this chapter, we describe a detailed protocol that is optimized for mouse embryonic stem cells. The result of this differentiation is a homogenous adipocyte monolayer culture that can be used for several applications including developmental and pharmacological research.",
keywords = "Adipocyte differentiation, Ascorbic acid, Monolayer culture, Mouse embryonic stem cell",
author = "Ixchelt Cuaranta-Monroy and Zoltan Simandi and Laszlo Nagy",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2015.",
year = "2016",
month = oct,
day = "1",
doi = "10.1007/7651_2015_219",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "407--415",
booktitle = "Methods in Molecular Biology",
}