Differential transport of DNA by a rectified Brownian motion device

Richard W. Hammond; Joel S. Bader; Steven A. Henck; Michael W. Deem; Gregory A. McDermott; James M. Bustillo; Jonathan M. Rothberg

doi:10.1002/(SICI)1522-2683(20000101)21:1<74::AID-ELPS74>3.0.CO;2-K

Differential transport of DNA by a rectified Brownian motion device

Richard W. Hammond, Joel S. Bader, Steven A. Henck, Michael W. Deem, Gregory A. McDermott, James M. Bustillo, Jonathan M. Rothberg

Research output: Contribution to journal › Article › peer-review

Abstract

An interdigitated electrode array (IDEA) device has been designed and used to transport DNA based on a Brownian ratchet mechanism. This migration is produced by the periodic formation of an asymmetric sawtooth electric field in the device. Oligonucleotides of 25, 50, end 100 bases in length were tested using two different array geometries. DNA transport as a function of DNA size, electric field frequency, and array geometry is shown to be in qualitative agreement with theory. Such a device could provide for DNA separations over a broad size range, and can be readily scaled as a component in a microfabricated DNA analysis system.

Original language	English (US)
Pages (from-to)	74-80
Number of pages	7
Journal	ELECTROPHORESIS
Volume	21
Issue number	1
DOIs	https://doi.org/10.1002/(SICI)1522-2683(20000101)21:1<74::AID-ELPS74>3.0.CO;2-K
State	Published - 2000
Externally published	Yes

Keywords

Brownian ratchet
DNA
Microdevices
Microfabrication

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

Access to Document

10.1002/(SICI)1522-2683(20000101)21:1<74::AID-ELPS74>3.0.CO;2-K

Cite this

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title = "Differential transport of DNA by a rectified Brownian motion device",

abstract = "An interdigitated electrode array (IDEA) device has been designed and used to transport DNA based on a Brownian ratchet mechanism. This migration is produced by the periodic formation of an asymmetric sawtooth electric field in the device. Oligonucleotides of 25, 50, end 100 bases in length were tested using two different array geometries. DNA transport as a function of DNA size, electric field frequency, and array geometry is shown to be in qualitative agreement with theory. Such a device could provide for DNA separations over a broad size range, and can be readily scaled as a component in a microfabricated DNA analysis system.",

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T1 - Differential transport of DNA by a rectified Brownian motion device

AU - Hammond, Richard W.

AU - Bader, Joel S.

AU - Henck, Steven A.

AU - Deem, Michael W.

AU - McDermott, Gregory A.

AU - Bustillo, James M.

AU - Rothberg, Jonathan M.

PY - 2000

Y1 - 2000

N2 - An interdigitated electrode array (IDEA) device has been designed and used to transport DNA based on a Brownian ratchet mechanism. This migration is produced by the periodic formation of an asymmetric sawtooth electric field in the device. Oligonucleotides of 25, 50, end 100 bases in length were tested using two different array geometries. DNA transport as a function of DNA size, electric field frequency, and array geometry is shown to be in qualitative agreement with theory. Such a device could provide for DNA separations over a broad size range, and can be readily scaled as a component in a microfabricated DNA analysis system.

AB - An interdigitated electrode array (IDEA) device has been designed and used to transport DNA based on a Brownian ratchet mechanism. This migration is produced by the periodic formation of an asymmetric sawtooth electric field in the device. Oligonucleotides of 25, 50, end 100 bases in length were tested using two different array geometries. DNA transport as a function of DNA size, electric field frequency, and array geometry is shown to be in qualitative agreement with theory. Such a device could provide for DNA separations over a broad size range, and can be readily scaled as a component in a microfabricated DNA analysis system.

KW - Brownian ratchet

KW - DNA

KW - Microdevices

KW - Microfabrication

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Differential transport of DNA by a rectified Brownian motion device

Abstract

Keywords

ASJC Scopus subject areas

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