Differential requirements for COPI transport during vertebrate early development

Pedro Coutinho, Michael J. Parsons, Kevin A. Thomas, Elizabeth M.A. Hirst, Leonor Saúde, Isabel Campos, P. Huw Williams, Derek L. Stemple

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


The coatomer vesicular coat complex is essential for normal Golgi and secretory activities in eukaryotic cells. Through positional cloning of genes controlling zebrafish notochord development, we found that the sneezy, happy, and dopey loci encode the α, β, and β′ subunits of the coatomer complex. Export from mutant endoplasmic reticulum is blocked, Golgi structure is disrupted, and mutant embryos eventually degenerate due to widespread apoptosis. The early embryonic phenotype, however, demonstrates that despite its "housekeeping" functions, coatomer activity is specifically and cell autonomously required for normal chordamesoderm differentiation, perinotochordal basement membrane formation, and melanophore pigmentation. Hence, differential requirements for coatomer activity among embryonic tissues lead to tissue-specific developmental defects. Moreover, we note that the mRNA encoding α coatomer is strikingly upregulated in notochord progenitors, and we present data suggesting that α coatomer transcription is tuned to activity- and cell type-specific secretory loads.

Original languageEnglish (US)
Pages (from-to)547-558
Number of pages12
JournalDevelopmental Cell
Issue number4
StatePublished - Oct 2004
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology


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