Differential expression patterns of phospholipase D isoforms 1 and 2 in the mammalian brain and retina

Casey N. Barber, Hana L. Goldschmidt, Brendan Lilley, Alexei M. Bygrave, Richard C. Johnson, Richard L. Huganir, Donald J. Zack, Daniel M. Raben

Research output: Contribution to journalArticlepeer-review

Abstract

Phosphatidic acid is a key signaling molecule heavily implicated in exocytosis due to its protein-binding partners and propensity to induce negative membrane curvature. One phosphatidic acid-producing enzyme, phospholipase D (PLD), has also been implicated in neurotransmission. Unfortunately, due to the unreliability of reagents, there has been confusion in the literature regarding the expression of PLD isoforms in the mammalian brain which has hampered our understanding of their functional roles in neurons. To address this, we generated epitope-tagged PLD1 and PLD2 knockin mice using CRISPR/Cas9. Using these mice, we show that PLD1 and PLD2 are both localized at synapses by adulthood, with PLD2 expression being considerably higher in glial cells and PLD1 expression predominating in neurons. Interestingly, we observed that only PLD1 is expressed in the mouse retina, where it is found in the synaptic plexiform layers. These data provide critical information regarding the localization and potential role of PLDs in the central nervous system.

Original languageEnglish (US)
Article number100247
JournalJournal of Lipid Research
Volume63
Issue number8
DOIs
StatePublished - Aug 2022

Keywords

  • CRISPR/Cas9
  • Supplementary key words phospholipids/phosphatidic acid
  • brain lipids
  • exocytosis
  • eye/retina
  • glia
  • knock-in mice
  • neurotransmission
  • phospholipases/D
  • synaptic plexiform layer

ASJC Scopus subject areas

  • Endocrinology
  • Biochemistry
  • Cell Biology

Fingerprint

Dive into the research topics of 'Differential expression patterns of phospholipase D isoforms 1 and 2 in the mammalian brain and retina'. Together they form a unique fingerprint.

Cite this