TY - JOUR
T1 - Differential expression of aquaporin-4 isoforms localizes with neuromyelitis optica disease activity
AU - Saini, Harleen
AU - Fernandez, Gregory
AU - Kerr, Douglas
AU - Levy, Michael
N1 - Funding Information:
Human brain, spinal cord and optic nerve tissue from twelve de-identified controls were obtained through the National Institute of Child Health and Development Brain and Tissue Bank at the University of Maryland (Baltimore, MD, contract HHSN275200900011C, Ref. No. N01-HD-9-0011) and from the Tissue Bank at the Rocky Mountain Multiple Sclerosis Center (Englewood, CO). These control subjects died from various causes not including neurologic diseases such as NMO or MS and their CNS tissue was harvested from 2–16 h post-mortem, flash frozen in liquid nitrogen and stored at − 80 °C. Purified cDNA from non-CNS human tissues was obtained from Origene (heart, spleen, liver, and leukocytes) and Clontech (placenta, pancreas, kidney, skeletal muscle, and lung).
Funding Information:
We would like to thank Peter Agre and Kathleen Burns for the intellectual input. This project has been generously funded by the Guthy Jackson Foundation , the American Academy of Neurology and Johns Hopkins Project RESTORE . We would also like to acknowledge the Maryland Brain and Tissue Bank at the University of Maryland for access to normal human tissue and Rocky Mountain Multiple Sclerosis Tissue Bank for access to normal human tissue and tissue from a patient with neuromyelitis optica.
PY - 2010/4
Y1 - 2010/4
N2 - Neuromyelitis optica (NMO) is a devastating neuroinflammatory disorder that specifically targets the spinal cord and optic nerves. Aquaporin-4 (AQP4) is the target of the NMO-IgG biomarker. AQP4 is expressed as two isoforms: M1 and M23, which have different functions in the central nervous system (CNS). We characterized the expression pattern of these AQP4 isoform mRNAs in humans and found a pattern of AQP4 expression that correlates with NMO disease localization. The ratio of M1:M23 mRNA is highest in the optic nerve and spinal cord, followed by brainstem, then the cerebral and cerebellar cortices.
AB - Neuromyelitis optica (NMO) is a devastating neuroinflammatory disorder that specifically targets the spinal cord and optic nerves. Aquaporin-4 (AQP4) is the target of the NMO-IgG biomarker. AQP4 is expressed as two isoforms: M1 and M23, which have different functions in the central nervous system (CNS). We characterized the expression pattern of these AQP4 isoform mRNAs in humans and found a pattern of AQP4 expression that correlates with NMO disease localization. The ratio of M1:M23 mRNA is highest in the optic nerve and spinal cord, followed by brainstem, then the cerebral and cerebellar cortices.
KW - Aquaporin-4
KW - Aquaporin-4 isoforms M1 and M23
KW - Astrocytes
KW - Neuromyelitis optica
KW - Rapid amplification of DNA ends (RACE)
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U2 - 10.1016/j.jneuroim.2010.02.007
DO - 10.1016/j.jneuroim.2010.02.007
M3 - Article
C2 - 20227773
AN - SCOPUS:77951229188
SN - 0165-5728
VL - 221
SP - 68
EP - 72
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -