TY - JOUR
T1 - Developments and applications of functional protein microarrays
AU - Syu, Guan Da
AU - Dunn, Jessica
AU - Zhu, Heng
N1 - Funding Information:
This work was supported in part by the NIH/NCI IMAT R33-CA186790-01A1, MOST Taiwan fellowship 105-2917-I-564-078, and MOST Taiwan 108-2320-B-006-054-MY2. JD was supported in part by the CBI program (NIH 2T32GM080189-11) at The Johns Hopkins University. The authors declare that they have no conflicts of interest with the contents of this article.
Funding Information:
* This work was supported in part by the NIH/NCI IMAT R33-CA186790-01A1, MOST Taiwan fellowship 105-2917-I-564-078, and MOST Taiwan 108-2320-B-006-054-MY2. JD was supported in part by the CBI program (NIH 2T32GM080189-11) at The Johns Hopkins University. The authors declare that they have no conflicts of interest with the contents of this article. ** To whom correspondence may be addressed: Room 327, Edward D. Miller Research Building, 733 N. Broadway, Baltimore, MD 21205. Tel.: +1-410-502-0878; Fax: +1-410-502-1872; E-mail: [email protected]. ‡‡ To whom correspondence may be addressed: Room 89A07, Department of Biotechnology and Bioindustry Sciences, No.1, University Road, Tainan City 701, Taiwan (R.O.C). Tel.: +886-6-275-7575#58231; Fax: +886-6-276-6490; E-mail: [email protected]. Author contributions: G.-D.S., J.D., and H.Z. wrote the paper.
Publisher Copyright:
© 2020 Syu et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.
PY - 2020/6
Y1 - 2020/6
N2 - Protein microarrays are crucial tools in the study of proteins in an unbiased, high-throughput manner, as they allow for characterization of up to thousands of individually purified proteins in parallel. The adaptability of this technology has enabled its use in a wide variety of applications, including the study of proteome-wide molecular interactions, analysis of post-translational modifications, identification of novel drug targets, and examination of pathogen-host interactions. In addition, the technology has also been shown to be useful in profiling antibody specificity, as well as in the discovery of novel biomarkers, especially for autoimmune diseases and cancers. In this review, we will summarize the developments that have been made in protein microarray technology in both in basic and translational research over the past decade. We will also introduce a novel membrane protein array, the GPCR-VirD array, and discuss the future directions of functional protein microarrays.
AB - Protein microarrays are crucial tools in the study of proteins in an unbiased, high-throughput manner, as they allow for characterization of up to thousands of individually purified proteins in parallel. The adaptability of this technology has enabled its use in a wide variety of applications, including the study of proteome-wide molecular interactions, analysis of post-translational modifications, identification of novel drug targets, and examination of pathogen-host interactions. In addition, the technology has also been shown to be useful in profiling antibody specificity, as well as in the discovery of novel biomarkers, especially for autoimmune diseases and cancers. In this review, we will summarize the developments that have been made in protein microarray technology in both in basic and translational research over the past decade. We will also introduce a novel membrane protein array, the GPCR-VirD array, and discuss the future directions of functional protein microarrays.
UR - http://www.scopus.com/inward/record.url?scp=85085714639&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85085714639&partnerID=8YFLogxK
U2 - 10.1074/mcp.r120.001936
DO - 10.1074/mcp.r120.001936
M3 - Review article
C2 - 32303587
AN - SCOPUS:85085714639
SN - 1535-9476
VL - 19
SP - 916
EP - 927
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
IS - 6
ER -