Developmental toxicity and brain aromatase induction by high genistein concentrations in zebrafish embryos

Dong Jae Kim; Seung Hyeok Seok; Min Won Baek; Hui Young Lee; Yi Rang Na; Sung Hoon Park; Hyun Kyoung Lee; Noton Kumar Dutta; Koichi Kawakami; Jae Hak Park

doi:10.1080/15376510802563330

Developmental toxicity and brain aromatase induction by high genistein concentrations in zebrafish embryos

Dong Jae Kim, Seung Hyeok Seok, Min Won Baek, Hui Young Lee, Yi Rang Na, Sung Hoon Park, Hyun Kyoung Lee, Noton Kumar Dutta, Koichi Kawakami, Jae Hak Park

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Genistein is a phytoestrogen found at a high level in soybeans. In vitro and in vivo studies showed that high concentrations of genistein caused toxic effects. This study was designed to test the feasibility of zebrafish embryos for evaluating developmental toxicity and estrogenic potential of high genistein concentrations. The zebrafish embryos at 24 h post-fertilization were exposed to genistein (1 3 10^-4 M, 0.5 3 10^-4 M, 0.25 3 10 ^-4 M) or vehicle (ethanol, 0.1%) for 60 h. Genistein-treated embryos showed decreased heart rates, retarded hatching times, decreased body length, and increased mortality in a dose-dependent manner. After 0.25 3 10^-4 M genistein treatment, malformations of survived embryos such as pericardial edema, yolk sac edema, and spinal kyphosis were also observed. TUNEL assay results showed apoptotic DNA fragments in brain. This study also confirmed the estrogenic potential of genistein by EGFP expression in the brain of the mosaic reporter zebrafish embryos. This study first demonstrated that high concentrations of genistein caused a teratogenic effect on zebrafish embryos and confirmed the estrogenic potential of genistein in mosaic reporter zebrafish embryos.

Original language	English (US)
Pages (from-to)	251-256
Number of pages	6
Journal	Toxicology Mechanisms and Methods
Volume	19
Issue number	3
DOIs	https://doi.org/10.1080/15376510802563330
State	Published - 2009
Externally published	Yes

Keywords

Brain aromatase
Developmental toxicity
EGFP
Genistein
Zebrafish embryo

ASJC Scopus subject areas

Toxicology
Health, Toxicology and Mutagenesis

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10.1080/15376510802563330

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title = "Developmental toxicity and brain aromatase induction by high genistein concentrations in zebrafish embryos",

abstract = "Genistein is a phytoestrogen found at a high level in soybeans. In vitro and in vivo studies showed that high concentrations of genistein caused toxic effects. This study was designed to test the feasibility of zebrafish embryos for evaluating developmental toxicity and estrogenic potential of high genistein concentrations. The zebrafish embryos at 24 h post-fertilization were exposed to genistein (1 3 10-4 M, 0.5 3 10-4 M, 0.25 3 10 -4 M) or vehicle (ethanol, 0.1%) for 60 h. Genistein-treated embryos showed decreased heart rates, retarded hatching times, decreased body length, and increased mortality in a dose-dependent manner. After 0.25 3 10-4 M genistein treatment, malformations of survived embryos such as pericardial edema, yolk sac edema, and spinal kyphosis were also observed. TUNEL assay results showed apoptotic DNA fragments in brain. This study also confirmed the estrogenic potential of genistein by EGFP expression in the brain of the mosaic reporter zebrafish embryos. This study first demonstrated that high concentrations of genistein caused a teratogenic effect on zebrafish embryos and confirmed the estrogenic potential of genistein in mosaic reporter zebrafish embryos.",

keywords = "Brain aromatase, Developmental toxicity, EGFP, Genistein, Zebrafish embryo",

author = "Kim, {Dong Jae} and Seok, {Seung Hyeok} and Baek, {Min Won} and Lee, {Hui Young} and Na, {Yi Rang} and Park, {Sung Hoon} and Lee, {Hyun Kyoung} and Dutta, {Noton Kumar} and Koichi Kawakami and Park, {Jae Hak}",

note = "Funding Information: This work was supported by the Korea Research Foundation grants (KRF-005-E00077), the BK21 Program for Veterinary Science and the Research Institute for Veterinary Science, Seoul National University, Korea.",

year = "2009",

doi = "10.1080/15376510802563330",

language = "English (US)",

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T1 - Developmental toxicity and brain aromatase induction by high genistein concentrations in zebrafish embryos

AU - Kim, Dong Jae

AU - Seok, Seung Hyeok

AU - Baek, Min Won

AU - Lee, Hui Young

AU - Na, Yi Rang

AU - Park, Sung Hoon

AU - Lee, Hyun Kyoung

AU - Dutta, Noton Kumar

AU - Kawakami, Koichi

AU - Park, Jae Hak

N1 - Funding Information: This work was supported by the Korea Research Foundation grants (KRF-005-E00077), the BK21 Program for Veterinary Science and the Research Institute for Veterinary Science, Seoul National University, Korea.

PY - 2009

Y1 - 2009

N2 - Genistein is a phytoestrogen found at a high level in soybeans. In vitro and in vivo studies showed that high concentrations of genistein caused toxic effects. This study was designed to test the feasibility of zebrafish embryos for evaluating developmental toxicity and estrogenic potential of high genistein concentrations. The zebrafish embryos at 24 h post-fertilization were exposed to genistein (1 3 10-4 M, 0.5 3 10-4 M, 0.25 3 10 -4 M) or vehicle (ethanol, 0.1%) for 60 h. Genistein-treated embryos showed decreased heart rates, retarded hatching times, decreased body length, and increased mortality in a dose-dependent manner. After 0.25 3 10-4 M genistein treatment, malformations of survived embryos such as pericardial edema, yolk sac edema, and spinal kyphosis were also observed. TUNEL assay results showed apoptotic DNA fragments in brain. This study also confirmed the estrogenic potential of genistein by EGFP expression in the brain of the mosaic reporter zebrafish embryos. This study first demonstrated that high concentrations of genistein caused a teratogenic effect on zebrafish embryos and confirmed the estrogenic potential of genistein in mosaic reporter zebrafish embryos.

AB - Genistein is a phytoestrogen found at a high level in soybeans. In vitro and in vivo studies showed that high concentrations of genistein caused toxic effects. This study was designed to test the feasibility of zebrafish embryos for evaluating developmental toxicity and estrogenic potential of high genistein concentrations. The zebrafish embryos at 24 h post-fertilization were exposed to genistein (1 3 10-4 M, 0.5 3 10-4 M, 0.25 3 10 -4 M) or vehicle (ethanol, 0.1%) for 60 h. Genistein-treated embryos showed decreased heart rates, retarded hatching times, decreased body length, and increased mortality in a dose-dependent manner. After 0.25 3 10-4 M genistein treatment, malformations of survived embryos such as pericardial edema, yolk sac edema, and spinal kyphosis were also observed. TUNEL assay results showed apoptotic DNA fragments in brain. This study also confirmed the estrogenic potential of genistein by EGFP expression in the brain of the mosaic reporter zebrafish embryos. This study first demonstrated that high concentrations of genistein caused a teratogenic effect on zebrafish embryos and confirmed the estrogenic potential of genistein in mosaic reporter zebrafish embryos.

KW - Brain aromatase

KW - Developmental toxicity

KW - EGFP

KW - Genistein

KW - Zebrafish embryo

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Developmental toxicity and brain aromatase induction by high genistein concentrations in zebrafish embryos

Abstract

Keywords

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