Developmental role of GnRH and PACAP in a zebrafish model

Nancy M. Sherwood, Sheng Wu

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


GnRH is expressed early in development long before reproduction begins. To determine whether GnRH has a role in development, gene knockdown with morpholinos was used in one-cell zebrafish embryos to block translation of gnrh mRNA into protein. Gene knockdown of gnrh2, gnrh3 or both at the one-cell stage resulted in a high percentage of embryos at 24-48 h with a defective mid-hindbrain boundary and underdeveloped eyes; a small percentage of embryos at 72 h had a defective heart. In similar studies on GHRH-PACAP, gene knockdown resulted in a smaller brain and eyes, but a normal-appearing heart. The evidence supports a role for the three neuropeptides in early development.

Original languageEnglish (US)
Pages (from-to)74-80
Number of pages7
JournalGeneral and Comparative Endocrinology
Issue number1-2 SPEC. ISS.
StatePublished - May 15 2005
Externally publishedYes


  • Brain
  • Development
  • Fish
  • GHRH
  • Gene knockdown
  • GnRH
  • Morpholino
  • Neuropeptides
  • Reproduction
  • Zebrafish

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology


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