Abstract
Human pluripotent stem cells (PSCs), in particular patient-derived induced pluripotent stem cells (iPSCs), provide a unique cell resource for various applications in regenerative medicine, including disease modeling, drug discovery, and cell replacement therapy. Highly efficient approaches that differentiate PSCs into functional neurons, as well as other neural cells, are critical for modeling neurological disorders and testing potential therapies. Transcription factors are master regulators of cell identity. Transcription factor-driven strategies for cell differentiation and transdifferentiation have emerged as a robust platform that can rapidly generate highly pure and functional neurons and glia from PSCs and somatic cells. In this chapter, we focus on the development of transcription factor-based strategies for generating various neuron subtypes from PSCs and somatic cells. In addition, we discuss key elements for consideration when optimizing transcription factor-driven strategies for neural differentiation, such as the choice of transcription factor drivers and methods for transcription factor delivery.
Original language | English (US) |
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Title of host publication | Phenotyping of Human iPSC-derived Neurons |
Subtitle of host publication | Patient-Driven Research |
Publisher | Elsevier |
Pages | 73-96 |
Number of pages | 24 |
ISBN (Electronic) | 9780128222775 |
ISBN (Print) | 9780128222782 |
DOIs | |
State | Published - Jan 1 2022 |
Keywords
- Astrocyte
- bHLH transcription factors
- Cell identity changes
- Cholinergic neuron
- Dopaminergic neuron
- Embryonic stem cells
- GABAergic neuron
- Glutamatergic neuron
- Induced pluripotent stem cells
- Oligodendrocyte
- Rapid differentiation
- Transdifferentiation
ASJC Scopus subject areas
- General Medicine
- General Neuroscience