Development of an operator-independent method for seeding tissue-engineered vascular grafts

Brooks Udelsman; Narutoshi Hibino; Gustavo A. Villalona; Edward McGillicuddy; Alejandro Nieponice; Yuki Sakamoto; Shojiro Matsuda; David A. Vorp; Toshiharu Shinoka; Christopher K. Breuer

doi:10.1089/ten.tec.2010.0581

Development of an operator-independent method for seeding tissue-engineered vascular grafts

Brooks Udelsman, Narutoshi Hibino, Gustavo A. Villalona, Edward McGillicuddy, Alejandro Nieponice, Yuki Sakamoto, Shojiro Matsuda, David A. Vorp, Toshiharu Shinoka, Christopher K. Breuer

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The manual seeding of cells onto a biodegradable scaffold by pipetting is an effective method of cell seeding. However, the widespread use and ultimate clinical utility of this technique is limited by operator variability. This study was conducted to evaluate an operator-independent vacuum-seeding method for use in an upcoming clinical trial. Using bone marrow-derived mononuclear cells, we achieved seeding comparable to manually seeded scaffolds in terms of cellular attachment, distribution, and viability in vacuum-seeded grafts at vacuum pressures of -25 to -50mmHg. In conclusion, we describe an operator-independent seeding method for use in the clinical setting.

Original language	English (US)
Pages (from-to)	731-736
Number of pages	6
Journal	Tissue Engineering - Part C: Methods
Volume	17
Issue number	7
DOIs	https://doi.org/10.1089/ten.tec.2010.0581
State	Published - Jul 1 2011
Externally published	Yes

ASJC Scopus subject areas

Medicine (miscellaneous)
Bioengineering
Biomedical Engineering

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abstract = "The manual seeding of cells onto a biodegradable scaffold by pipetting is an effective method of cell seeding. However, the widespread use and ultimate clinical utility of this technique is limited by operator variability. This study was conducted to evaluate an operator-independent vacuum-seeding method for use in an upcoming clinical trial. Using bone marrow-derived mononuclear cells, we achieved seeding comparable to manually seeded scaffolds in terms of cellular attachment, distribution, and viability in vacuum-seeded grafts at vacuum pressures of -25 to -50mmHg. In conclusion, we describe an operator-independent seeding method for use in the clinical setting.",

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AU - Udelsman, Brooks

AU - Hibino, Narutoshi

AU - Villalona, Gustavo A.

AU - McGillicuddy, Edward

AU - Nieponice, Alejandro

AU - Sakamoto, Yuki

AU - Matsuda, Shojiro

AU - Vorp, David A.

AU - Shinoka, Toshiharu

AU - Breuer, Christopher K.

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Development of an operator-independent method for seeding tissue-engineered vascular grafts

Abstract

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