Desarrollo de un método de electroinmunotransferencia para la detección de anticuerpos anti sarcocystis aucheniae en alpacas

T. Rosa Sam; Z. Armando González; U. Teresa López; P. Manuela Verástegui

Desarrollo de un método de electroinmunotransferencia para la detección de anticuerpos anti sarcocystis aucheniae en alpacas

Translated title of the contribution: Development of a Western blot method for the detection of antibodies against Sarcocystis aucheniae in alpacas

T. Rosa Sam, Z. Armando González, U. Teresa López, P. Manuela Verástegui

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A western blot assay to detect antibodies against Sarcocystis aucheniae (SA) from alpacas was developed for in vivo diagnosis of sarcocystiosis in domestic camelids. The antigen consists of a soluble extract obtained from SA macrocysts (MSA) of naturally infected alpacas. A SDS-PAGE (15%) electrophoretic analysis revealed fifteen peptides at 25-127 kDa. Western blot assay ofsera from MSA immunized rabbits detected three peptides at 58-50 KDa, while the sera from SA diagnosed alpacas reacted against four protein bands. Sera from neonate alpacas and from non-SA infected alpacas did not display reactivity to the MSA peptides. Specific immune reactivity ofthe alpaca antibodies against SA with four MSA peptides by a western blot assay has been documented.

Translated title of the contribution	Development of a Western blot method for the detection of antibodies against Sarcocystis aucheniae in alpacas
Original language	Spanish
Pages (from-to)	82-85
Number of pages	4
Journal	Revista de Investigaciones Veterinarias del Peru
Volume	10
Issue number	2
State	Published - 1999
Externally published	Yes

Keywords

Antigen
Macrocysts
Western blot

ASJC Scopus subject areas

General Veterinary

Cite this

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title = "Desarrollo de un m{\'e}todo de electroinmunotransferencia para la detecci{\'o}n de anticuerpos anti sarcocystis aucheniae en alpacas",

abstract = "A western blot assay to detect antibodies against Sarcocystis aucheniae (SA) from alpacas was developed for in vivo diagnosis of sarcocystiosis in domestic camelids. The antigen consists of a soluble extract obtained from SA macrocysts (MSA) of naturally infected alpacas. A SDS-PAGE (15%) electrophoretic analysis revealed fifteen peptides at 25-127 kDa. Western blot assay ofsera from MSA immunized rabbits detected three peptides at 58-50 KDa, while the sera from SA diagnosed alpacas reacted against four protein bands. Sera from neonate alpacas and from non-SA infected alpacas did not display reactivity to the MSA peptides. Specific immune reactivity ofthe alpaca antibodies against SA with four MSA peptides by a western blot assay has been documented.",

keywords = "Antigen, Macrocysts, Western blot",

author = "{Rosa Sam}, T. and {Armando Gonz{\'a}lez}, Z. and {Teresa L{\'o}pez}, U. and {Manuela Ver{\'a}stegui}, P.",

year = "1999",

language = "Spanish",

volume = "10",

pages = "82--85",

journal = "Revista de Investigaciones Veterinarias del Peru",

issn = "1682-3419",

publisher = "Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos",

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T1 - Desarrollo de un método de electroinmunotransferencia para la detección de anticuerpos anti sarcocystis aucheniae en alpacas

AU - Rosa Sam, T.

AU - Armando González, Z.

AU - Teresa López, U.

AU - Manuela Verástegui, P.

PY - 1999

Y1 - 1999

N2 - A western blot assay to detect antibodies against Sarcocystis aucheniae (SA) from alpacas was developed for in vivo diagnosis of sarcocystiosis in domestic camelids. The antigen consists of a soluble extract obtained from SA macrocysts (MSA) of naturally infected alpacas. A SDS-PAGE (15%) electrophoretic analysis revealed fifteen peptides at 25-127 kDa. Western blot assay ofsera from MSA immunized rabbits detected three peptides at 58-50 KDa, while the sera from SA diagnosed alpacas reacted against four protein bands. Sera from neonate alpacas and from non-SA infected alpacas did not display reactivity to the MSA peptides. Specific immune reactivity ofthe alpaca antibodies against SA with four MSA peptides by a western blot assay has been documented.

AB - A western blot assay to detect antibodies against Sarcocystis aucheniae (SA) from alpacas was developed for in vivo diagnosis of sarcocystiosis in domestic camelids. The antigen consists of a soluble extract obtained from SA macrocysts (MSA) of naturally infected alpacas. A SDS-PAGE (15%) electrophoretic analysis revealed fifteen peptides at 25-127 kDa. Western blot assay ofsera from MSA immunized rabbits detected three peptides at 58-50 KDa, while the sera from SA diagnosed alpacas reacted against four protein bands. Sera from neonate alpacas and from non-SA infected alpacas did not display reactivity to the MSA peptides. Specific immune reactivity ofthe alpaca antibodies against SA with four MSA peptides by a western blot assay has been documented.

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KW - Macrocysts

KW - Western blot

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AN - SCOPUS:84873443198

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JO - Revista de Investigaciones Veterinarias del Peru

JF - Revista de Investigaciones Veterinarias del Peru

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Desarrollo de un método de electroinmunotransferencia para la detección de anticuerpos anti sarcocystis aucheniae en alpacas

Abstract

Keywords

ASJC Scopus subject areas

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