Development of a Tethered mRNA Amplifier to increase protein expression

Bahareh Torkzaban, Ryan Kawalerski, Jeff Coller

Research output: Contribution to journalArticlepeer-review


Herein, we present a novel method to specifically increase a messenger RNA's (mRNA) expression at the post-transcriptional level. This is accomplished using what we term a “Tethered mRNA Amplifier.” The Tethered mRNA Amplifier specifically binds an mRNA's 3′ untranslated region and enhances its stability/translation, often doubling protein output. We test this approach on several transcripts associated with haploinsufficiency disorders and increase their steady-state expression in cell culture. We suggest this approach may be a tenable therapeutic modality with precise activity and broad-spectrum application.

Original languageEnglish (US)
Article number2200214
JournalBiotechnology Journal
Issue number10
StatePublished - Oct 2022


  • gene expression
  • haploinsufficiency
  • mRNA metabolism
  • mRNA therapeutics
  • mRNA translation

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine


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