Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy

Christina M. Scheel; Sukwan Handali; Edneia C. Bueno; Azra Khan; Kathy Hancock; Armando E. Gonzalez; Hector H. Garcia; Robert H. Gilman; Victor C.W. Tsang

doi:10.1080/15321810600573028

Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy

Christina M. Scheel, Sukwan Handali, Edneia C. Bueno, Azra Khan, Kathy Hancock, Armando E. Gonzalez, Hector H. Garcia, Robert H. Gilman, Victor C.W. Tsang

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Internal standard reference curves are used in enzyme-linked immunosorbent assay (ELISA) plates to control for inter- and intra-assay variance. To compare the diagnostic potential of multiple T. solium antigens on an unbiased, universal scale, we have created a standard curve using normal, human immunoglobulin G (hIgG). The hIgG curve is inexpensive and simple to prepare, and remains stable at 5°C for at least one year, with a coefficient of variance of less than 10%. The hIgG standard curve has proven a critical tool for the comparison of several diagnostic antigens slated for assay development.

Original language	English (US)
Pages (from-to)	173-181
Number of pages	9
Journal	Journal of Immunoassay and Immunochemistry
Volume	27
Issue number	2
DOIs	https://doi.org/10.1080/15321810600573028
State	Published - Jul 1 2006

Keywords

Antigen efficacy
Assay development
Diagnosis
ELISA
Immunoglobulin G
Standard curve

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Clinical Biochemistry
Medical Laboratory Technology

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10.1080/15321810600573028

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Scheel, C. M., Handali, S., Bueno, E. C., Khan, A., Hancock, K., Gonzalez, A. E., Garcia, H. H., Gilman, R. H., & Tsang, V. C. W. (2006). Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy. Journal of Immunoassay and Immunochemistry, 27(2), 173-181. https://doi.org/10.1080/15321810600573028

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AU - Scheel, Christina M.

AU - Handali, Sukwan

AU - Bueno, Edneia C.

AU - Khan, Azra

AU - Hancock, Kathy

AU - Gonzalez, Armando E.

AU - Garcia, Hector H.

AU - Gilman, Robert H.

AU - Tsang, Victor C.W.

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KW - Antigen efficacy

KW - Assay development

KW - Diagnosis

KW - ELISA

KW - Immunoglobulin G

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Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy

Abstract

Keywords

ASJC Scopus subject areas

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