Development of a hybrid EPR/NMR coimaging system

Alexandre Samouilov, George L. Caia, Eric Kesselring, Sergey Petryakov, Tomasz Wasowicz, Jay L. Zweier

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Electron paramagnetic resonance imaging (EPRI) is a powerful technique that enables spatial mapping of free radicals or other paramagnetic compounds; however, it does not in itself provide anatomic visualization of the body. Proton magnetic resonance imaging (MRI) is well suited to provide anatomical visualization. A hybrid EPR/NMR coimaging instrument was constructed that utilizes the complementary capabilities of both techniques, superimposing EPR and proton-MR images to provide the distribution of paramagnetic species in the body. A common magnet and field gradient system is utilized along with a dual EPR and proton-NMR resonator assembly, enabling coimaging without the need to move the sample. EPRI is performed at ∼1.2 GHz/ ∼40 mT and proton MRI is performed at 16.18 MHz/∼380 mT; hence the method is suitable for whole-body coimaging of living mice. The gradient system used is calibrated and controlled in such a manner that the spatial geometry of the two acquired images is matched, enabling their superposition without additional postprocessing or marker registration. The performance of the system was tested in a series of phantoms and in vivo applications by mapping the location of a paramagnetic probe in the gastrointestinal (GI) tract of mice. This hybrid EPR/NMR coimaging instrument enables imaging of paramagnetic molecules along with their anatomic localization in the body.

Original languageEnglish (US)
Pages (from-to)156-166
Number of pages11
JournalMagnetic Resonance in Medicine
Issue number1
StatePublished - Jul 2007
Externally publishedYes


  • EPR imaging
  • Free radicals, oxygen
  • Image coregistration
  • In vivo EPR
  • In vivo NMR
  • Proton MRI

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology


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