Development of a diagnostic polymerase chain reaction assay for detection of Mycoplasma hominis

Gary L. Gallia; Joseph M. Petroziello; Joseph M. Brogan; Ferne K. McCleskey; Vito G. DelVecchio

doi:10.1006/mcpr.1995.0064

Development of a diagnostic polymerase chain reaction assay for detection of Mycoplasma hominis

Gary L. Gallia, Joseph M. Petroziello, Joseph M. Brogan, Ferne K. McCleskey, Vito G. DelVecchio

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A polymerase chain reaction (PCR)-based assay was developed for the detection of Mycoplasma hominis. This assay generates a 152-bp PCR product which was part of an initial 471-bp M. hominis genomic DNA fragment. The 471-bp DNA fragment was shown by hybridization analysis to be unique for M. hominis. The PCR assay can amplify as few as 18 molecules of target DNA. This diagnostic assay offers potential for wide clinical application as it is rapid and can be successfully performed on crude sample preparations from a variety of media or biopsies. The use of this assay should aid in defining the aetiologic and pathologic roles played by M. hominis and thereby benefit patients.

Original language	English (US)
Pages (from-to)	415-421
Number of pages	7
Journal	Molecular and Cellular Probes
Volume	9
Issue number	6
DOIs	https://doi.org/10.1006/mcpr.1995.0064
State	Published - Jan 1 1995
Externally published	Yes

Keywords

DNA probe
Diagnostic PCR assay
Mycoplasma hominis

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1006/mcpr.1995.0064

Cite this

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title = "Development of a diagnostic polymerase chain reaction assay for detection of Mycoplasma hominis",

abstract = "A polymerase chain reaction (PCR)-based assay was developed for the detection of Mycoplasma hominis. This assay generates a 152-bp PCR product which was part of an initial 471-bp M. hominis genomic DNA fragment. The 471-bp DNA fragment was shown by hybridization analysis to be unique for M. hominis. The PCR assay can amplify as few as 18 molecules of target DNA. This diagnostic assay offers potential for wide clinical application as it is rapid and can be successfully performed on crude sample preparations from a variety of media or biopsies. The use of this assay should aid in defining the aetiologic and pathologic roles played by M. hominis and thereby benefit patients.",

keywords = "DNA probe, Diagnostic PCR assay, Mycoplasma hominis",

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note = "Funding Information: This work was supported by Air Force Office of Scientific Research Grants to G.L.G. and V.G.D.",

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AU - Gallia, Gary L.

AU - Petroziello, Joseph M.

AU - Brogan, Joseph M.

AU - McCleskey, Ferne K.

AU - DelVecchio, Vito G.

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Development of a diagnostic polymerase chain reaction assay for detection of Mycoplasma hominis

Abstract

Keywords

ASJC Scopus subject areas

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