Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis

Gabriel M.C. Barros, Dionísio D.A. Carvalho, Agnaldo S. Cruz, Ellen K.L. Morais, Ana Isabela L. Sales-Moioli, Talita K.B. Pinto, Melise C.D. Almeida, Ignacio Sanchez-Gendriz, Felipe Fernandes, Ingridy M.P. Barbalho, João P.Q. Santos, Jorge M.O. Henriques, César A.D. Teixeira, Paulo Gil, Lúcio Gama, Angélica E. Miranda, Karilany D. Coutinho, Leonardo J. Galvão-Lima, Ricardo A.M. Valentim

Research output: Contribution to journalArticlepeer-review

Abstract

The improvement of laboratory diagnosis is a critical step for the reduction of syphilis cases around the world. In this paper, we present the development of an impedance-based method for detecting T. pallidum antigens and antibodies as an auxiliary tool for syphilis laboratory diagnosis. We evaluate the voltammetric signal obtained after incubation in carbon or gold nanoparticle-modified carbon electrodes in the presence or absence of Poly-L-Lysine. Our results indicate that the signal obtained from the electrodes was sufficient to distinguish between infected and non-infected samples immediately (T0′) or 15 min (T15′) after incubation, indicating its potential use as a point-of-care method as a screening strategy.

Original languageEnglish (US)
Article number16206
JournalInternational journal of environmental research and public health
Volume19
Issue number23
DOIs
StatePublished - Dec 2022

Keywords

  • Treponema pallidum
  • electrodes
  • point-of-care method
  • screening strategy
  • syphilis

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Pollution
  • Health, Toxicology and Mutagenesis

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