Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis

Gabriel M.C. Barros; Dionísio D.A. Carvalho; Agnaldo S. Cruz; Ellen K.L. Morais; Ana Isabela L. Sales-Moioli; Talita K.B. Pinto; Melise C.D. Almeida; Ignacio Sanchez-Gendriz; Felipe Fernandes; Ingridy M.P. Barbalho; João P.Q. Santos; Jorge M.O. Henriques; César A.D. Teixeira; Paulo Gil; Lúcio Gama; Angélica E. Miranda; Karilany D. Coutinho; Leonardo J. Galvão-Lima; Ricardo A.M. Valentim

doi:10.3390/ijerph192316206

Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis

Gabriel M.C. Barros, Dionísio D.A. Carvalho, Agnaldo S. Cruz, Ellen K.L. Morais, Ana Isabela L. Sales-Moioli, Talita K.B. Pinto, Melise C.D. Almeida, Ignacio Sanchez-Gendriz, Felipe Fernandes, Ingridy M.P. Barbalho, João P.Q. Santos, Jorge M.O. Henriques, César A.D. Teixeira, Paulo Gil, Lúcio Gama, Angélica E. Miranda, Karilany D. Coutinho, Leonardo J. Galvão-Lima, Ricardo A.M. Valentim

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The improvement of laboratory diagnosis is a critical step for the reduction of syphilis cases around the world. In this paper, we present the development of an impedance-based method for detecting T. pallidum antigens and antibodies as an auxiliary tool for syphilis laboratory diagnosis. We evaluate the voltammetric signal obtained after incubation in carbon or gold nanoparticle-modified carbon electrodes in the presence or absence of Poly-L-Lysine. Our results indicate that the signal obtained from the electrodes was sufficient to distinguish between infected and non-infected samples immediately (T0′) or 15 min (T15′) after incubation, indicating its potential use as a point-of-care method as a screening strategy.

Original language	English (US)
Article number	16206
Journal	International journal of environmental research and public health
Volume	19
Issue number	23
DOIs	https://doi.org/10.3390/ijerph192316206
State	Published - Dec 2022

Keywords

Treponema pallidum
electrodes
point-of-care method
screening strategy
syphilis

ASJC Scopus subject areas

Pollution
Public Health, Environmental and Occupational Health
Health, Toxicology and Mutagenesis

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10.3390/ijerph192316206

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Barros, G. M. C., Carvalho, D. D. A., Cruz, A. S., Morais, E. K. L., Sales-Moioli, A. I. L., Pinto, T. K. B., Almeida, M. C. D., Sanchez-Gendriz, I., Fernandes, F., Barbalho, I. M. P., Santos, J. P. Q., Henriques, J. M. O., Teixeira, C. A. D., Gil, P., Gama, L., Miranda, A. E., Coutinho, K. D., Galvão-Lima, L. J., & Valentim, R. A. M. (2022). Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis. International journal of environmental research and public health, 19(23), Article 16206. https://doi.org/10.3390/ijerph192316206

Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis. / Barros, Gabriel M.C.; Carvalho, Dionísio D.A.; Cruz, Agnaldo S. et al.
In: International journal of environmental research and public health, Vol. 19, No. 23, 16206, 12.2022.

Research output: Contribution to journal › Article › peer-review

Barros, GMC, Carvalho, DDA, Cruz, AS, Morais, EKL, Sales-Moioli, AIL, Pinto, TKB, Almeida, MCD, Sanchez-Gendriz, I, Fernandes, F, Barbalho, IMP, Santos, JPQ, Henriques, JMO, Teixeira, CAD, Gil, P, Gama, L, Miranda, AE, Coutinho, KD, Galvão-Lima, LJ & Valentim, RAM 2022, 'Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis', International journal of environmental research and public health, vol. 19, no. 23, 16206. https://doi.org/10.3390/ijerph192316206

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abstract = "The improvement of laboratory diagnosis is a critical step for the reduction of syphilis cases around the world. In this paper, we present the development of an impedance-based method for detecting T. pallidum antigens and antibodies as an auxiliary tool for syphilis laboratory diagnosis. We evaluate the voltammetric signal obtained after incubation in carbon or gold nanoparticle-modified carbon electrodes in the presence or absence of Poly-L-Lysine. Our results indicate that the signal obtained from the electrodes was sufficient to distinguish between infected and non-infected samples immediately (T0′) or 15 min (T15′) after incubation, indicating its potential use as a point-of-care method as a screening strategy.",

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AU - Cruz, Agnaldo S.

AU - Morais, Ellen K.L.

AU - Sales-Moioli, Ana Isabela L.

AU - Pinto, Talita K.B.

AU - Almeida, Melise C.D.

AU - Sanchez-Gendriz, Ignacio

AU - Fernandes, Felipe

AU - Barbalho, Ingridy M.P.

AU - Santos, João P.Q.

AU - Henriques, Jorge M.O.

AU - Teixeira, César A.D.

AU - Gil, Paulo

AU - Gama, Lúcio

AU - Miranda, Angélica E.

AU - Coutinho, Karilany D.

AU - Galvão-Lima, Leonardo J.

AU - Valentim, Ricardo A.M.

PY - 2022/12

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Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis

Abstract

Keywords

ASJC Scopus subject areas

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