Detonation nanodiamond toxicity in human airway epithelial cells is modulated by air oxidation

Robert Silbajoris; William Linak; Olga Shenderova; Christopher Winterrowd; Huan Cheng Chang; Jay L. Zweier; Anirudh Kota; Lisa A. Dailey; Nicholas Nunn; Philip A. Bromberg; James M. Samet

doi:10.1016/j.diamond.2015.05.007

Detonation nanodiamond toxicity in human airway epithelial cells is modulated by air oxidation

Robert Silbajoris, William Linak, Olga Shenderova, Christopher Winterrowd, Huan Cheng Chang, Jay L. Zweier, Anirudh Kota, Lisa A. Dailey, Nicholas Nunn, Philip A. Bromberg, James M. Samet

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Detonation nanodiamonds (DNDs), a nanomaterial with an increasing range of industrial and biomedical applications, have previously been shown to induce a pro-inflammatory response in cultured human airway epithelial cells (HAECs). We now show that surface modifications induced by air oxidation of DND (AO-DND), including an increase in oxygen content, formation of carboxylic groups associated with the appearance of high negative zeta potential and a decrease in unpaired electron content, are accompanied by a significant loss of bioactivity, as measured by levels of interleukin-8 mRNA in HAEC. These findings are relevant to the identification of chemical determinants and molecular mechanisms of the inhalational toxicity of carbonaceous nanomaterials.

Original language	English (US)
Pages (from-to)	16-23
Number of pages	8
Journal	Diamond and Related Materials
Volume	58
DOIs	https://doi.org/10.1016/j.diamond.2015.05.007
State	Published - May 30 2015
Externally published	Yes

Keywords

Human airway epithelial cells
Interleukin-8
Nanodiamond
Toxicity
Zeta potential

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Materials Chemistry
Electrical and Electronic Engineering
Mechanical Engineering
Physics and Astronomy(all)
Chemistry(all)

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10.1016/j.diamond.2015.05.007

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title = "Detonation nanodiamond toxicity in human airway epithelial cells is modulated by air oxidation",

abstract = "Detonation nanodiamonds (DNDs), a nanomaterial with an increasing range of industrial and biomedical applications, have previously been shown to induce a pro-inflammatory response in cultured human airway epithelial cells (HAECs). We now show that surface modifications induced by air oxidation of DND (AO-DND), including an increase in oxygen content, formation of carboxylic groups associated with the appearance of high negative zeta potential and a decrease in unpaired electron content, are accompanied by a significant loss of bioactivity, as measured by levels of interleukin-8 mRNA in HAEC. These findings are relevant to the identification of chemical determinants and molecular mechanisms of the inhalational toxicity of carbonaceous nanomaterials.",

keywords = "Human airway epithelial cells, Interleukin-8, Nanodiamond, Toxicity, Zeta potential",

author = "Robert Silbajoris and William Linak and Olga Shenderova and Christopher Winterrowd and Chang, {Huan Cheng} and Zweier, {Jay L.} and Anirudh Kota and Dailey, {Lisa A.} and Nicholas Nunn and Bromberg, {Philip A.} and Samet, {James M.}",

year = "2015",

month = may,

day = "30",

doi = "10.1016/j.diamond.2015.05.007",

language = "English (US)",

volume = "58",

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journal = "Diamond and Related Materials",

issn = "0925-9635",

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TY - JOUR

T1 - Detonation nanodiamond toxicity in human airway epithelial cells is modulated by air oxidation

AU - Silbajoris, Robert

AU - Linak, William

AU - Shenderova, Olga

AU - Winterrowd, Christopher

AU - Chang, Huan Cheng

AU - Zweier, Jay L.

AU - Kota, Anirudh

AU - Dailey, Lisa A.

AU - Nunn, Nicholas

AU - Bromberg, Philip A.

AU - Samet, James M.

PY - 2015/5/30

Y1 - 2015/5/30

N2 - Detonation nanodiamonds (DNDs), a nanomaterial with an increasing range of industrial and biomedical applications, have previously been shown to induce a pro-inflammatory response in cultured human airway epithelial cells (HAECs). We now show that surface modifications induced by air oxidation of DND (AO-DND), including an increase in oxygen content, formation of carboxylic groups associated with the appearance of high negative zeta potential and a decrease in unpaired electron content, are accompanied by a significant loss of bioactivity, as measured by levels of interleukin-8 mRNA in HAEC. These findings are relevant to the identification of chemical determinants and molecular mechanisms of the inhalational toxicity of carbonaceous nanomaterials.

AB - Detonation nanodiamonds (DNDs), a nanomaterial with an increasing range of industrial and biomedical applications, have previously been shown to induce a pro-inflammatory response in cultured human airway epithelial cells (HAECs). We now show that surface modifications induced by air oxidation of DND (AO-DND), including an increase in oxygen content, formation of carboxylic groups associated with the appearance of high negative zeta potential and a decrease in unpaired electron content, are accompanied by a significant loss of bioactivity, as measured by levels of interleukin-8 mRNA in HAEC. These findings are relevant to the identification of chemical determinants and molecular mechanisms of the inhalational toxicity of carbonaceous nanomaterials.

KW - Human airway epithelial cells

KW - Interleukin-8

KW - Nanodiamond

KW - Toxicity

KW - Zeta potential

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SP - 16

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JO - Diamond and Related Materials

JF - Diamond and Related Materials

ER -

Detonation nanodiamond toxicity in human airway epithelial cells is modulated by air oxidation

Abstract

Keywords

ASJC Scopus subject areas

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