TY - JOUR
T1 - Determination of nitrosourea compounds in brain tissue by gas chromatography and electron capture detection
AU - Hassenbusch, Samuel J.
AU - Colvin, O. Michael
AU - Anderson, James H.
PY - 1995/7
Y1 - 1995/7
N2 - A relatively simple, high‐sensitivity gas chromatographic assay is described for nitrosourea compounds, such as BCNU [1,3‐bis(2‐chloroethyl)‐1‐nitrosourea] and MeCCNU [1‐(2‐chloroethyl)‐3‐(trans‐4‐methylcyclohexyl)‐1‐nitrosourea], in small biopsy samples of brain and other tissues. After extraction with ethyl acetate, secondary amines in BCNU and MeCCNU are derivatized with trifluoroacetic anhydride. Compounds are separated and quantitated by gas chromatography using a capillary column with temperature programming and an electron capture detector. Standard curves of BCNU indicate a coefficient of variance of 0.066 ± 0.018, a correlation coefficient of 0.929, and an extraction efficiency from whole brain of 68% with a minimum detectable amount of 20 ng in 5–10 mg samples. The assay has been facile and sensitive in over 1000 brain biopsy specimens after intravenous and intraarterial infusions of BCNU.
AB - A relatively simple, high‐sensitivity gas chromatographic assay is described for nitrosourea compounds, such as BCNU [1,3‐bis(2‐chloroethyl)‐1‐nitrosourea] and MeCCNU [1‐(2‐chloroethyl)‐3‐(trans‐4‐methylcyclohexyl)‐1‐nitrosourea], in small biopsy samples of brain and other tissues. After extraction with ethyl acetate, secondary amines in BCNU and MeCCNU are derivatized with trifluoroacetic anhydride. Compounds are separated and quantitated by gas chromatography using a capillary column with temperature programming and an electron capture detector. Standard curves of BCNU indicate a coefficient of variance of 0.066 ± 0.018, a correlation coefficient of 0.929, and an extraction efficiency from whole brain of 68% with a minimum detectable amount of 20 ng in 5–10 mg samples. The assay has been facile and sensitive in over 1000 brain biopsy specimens after intravenous and intraarterial infusions of BCNU.
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U2 - 10.1002/jps.2600840711
DO - 10.1002/jps.2600840711
M3 - Article
C2 - 7562434
AN - SCOPUS:0029014957
SN - 0022-3549
VL - 84
SP - 840
EP - 842
JO - Journal of Pharmaceutical Sciences
JF - Journal of Pharmaceutical Sciences
IS - 7
ER -