Determination of N-(phosphonacetyl)-L-aspartic acid (PALA) in plasma and urine by high pressure liquid chromatography

J. Lankelma; P. G M Penders; A. Leyva; H. M. Pinedo

Determination of N-(phosphonacetyl)-L-aspartic acid (PALA) in plasma and urine by high pressure liquid chromatography

J. Lankelma, P. G M Penders, A. Leyva, H. M. Pinedo

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Abstract

A rapid and sensitive determination of N-(phosphonacetyl)-L-aspartic acid (PALA) in plasma and urine using high pressure liquid chromatography is reported. A microparticulate, strong anion exchange column was used for the separation and PALA was detected by u.v. absorption at 205 nm. Interfering substances were removed from deproteinized plasma or from urine by a simple and quick clean-up procedure involving extraction with organic solvents. The detection limit for PALA was 5 x 10^-7M and linear dynamic range was greater than 10⁴. The assay time required was less than an hour. Plasma concentrations and urinary excretion of PALA were measured using this method in two patients treated with PALA. The ease and rapidity of this assay are advantages over reported methods and make possible close monitoring of the pharmacokinetics of this drug.

Original language	English (US)
Pages (from-to)	1483-1487
Number of pages	5
Journal	European Journal of Cancer and Clinical Oncology
Volume	16
Issue number	11
State	Published - 1980
Externally published	Yes

ASJC Scopus subject areas

Oncology

Cite this

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title = "Determination of N-(phosphonacetyl)-L-aspartic acid (PALA) in plasma and urine by high pressure liquid chromatography",

abstract = "A rapid and sensitive determination of N-(phosphonacetyl)-L-aspartic acid (PALA) in plasma and urine using high pressure liquid chromatography is reported. A microparticulate, strong anion exchange column was used for the separation and PALA was detected by u.v. absorption at 205 nm. Interfering substances were removed from deproteinized plasma or from urine by a simple and quick clean-up procedure involving extraction with organic solvents. The detection limit for PALA was 5 x 10-7M and linear dynamic range was greater than 104. The assay time required was less than an hour. Plasma concentrations and urinary excretion of PALA were measured using this method in two patients treated with PALA. The ease and rapidity of this assay are advantages over reported methods and make possible close monitoring of the pharmacokinetics of this drug.",

author = "J. Lankelma and Penders, {P. G M} and A. Leyva and Pinedo, {H. M.}",

year = "1980",

language = "English (US)",

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journal = "European Journal of Cancer and Clinical Oncology",

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T1 - Determination of N-(phosphonacetyl)-L-aspartic acid (PALA) in plasma and urine by high pressure liquid chromatography

AU - Lankelma, J.

AU - Penders, P. G M

AU - Leyva, A.

AU - Pinedo, H. M.

PY - 1980

Y1 - 1980

N2 - A rapid and sensitive determination of N-(phosphonacetyl)-L-aspartic acid (PALA) in plasma and urine using high pressure liquid chromatography is reported. A microparticulate, strong anion exchange column was used for the separation and PALA was detected by u.v. absorption at 205 nm. Interfering substances were removed from deproteinized plasma or from urine by a simple and quick clean-up procedure involving extraction with organic solvents. The detection limit for PALA was 5 x 10-7M and linear dynamic range was greater than 104. The assay time required was less than an hour. Plasma concentrations and urinary excretion of PALA were measured using this method in two patients treated with PALA. The ease and rapidity of this assay are advantages over reported methods and make possible close monitoring of the pharmacokinetics of this drug.

AB - A rapid and sensitive determination of N-(phosphonacetyl)-L-aspartic acid (PALA) in plasma and urine using high pressure liquid chromatography is reported. A microparticulate, strong anion exchange column was used for the separation and PALA was detected by u.v. absorption at 205 nm. Interfering substances were removed from deproteinized plasma or from urine by a simple and quick clean-up procedure involving extraction with organic solvents. The detection limit for PALA was 5 x 10-7M and linear dynamic range was greater than 104. The assay time required was less than an hour. Plasma concentrations and urinary excretion of PALA were measured using this method in two patients treated with PALA. The ease and rapidity of this assay are advantages over reported methods and make possible close monitoring of the pharmacokinetics of this drug.

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Determination of N-(phosphonacetyl)-L-aspartic acid (PALA) in plasma and urine by high pressure liquid chromatography

Abstract

ASJC Scopus subject areas

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