TY - JOUR
T1 - Determination of in vivo target search kinetics of regulatory noncoding RNA
AU - Fei, Jingyi
AU - Singh, Digvijay
AU - Zhang, Qiucen
AU - Park, Seongjin
AU - Balasubramanian, Divya
AU - Golding, Ido
AU - Vanderpool, Carin K.
AU - Ha, Taekjip
N1 - Publisher Copyright:
© 2015, American Association for the Advancement of Science. All rights reserved.
PY - 2015/3/20
Y1 - 2015/3/20
N2 - Base-pairing interactions between nucleic acids mediate target recognition in many biological processes.We developed a super-resolution imaging and modeling platform that enabled the in vivo determination of base pairing-mediated target recognition kinetics.We examined a stress-induced bacterial small RNA, SgrS, which induces the degradation of target messenger RNAs (mRNAs). SgrS binds to a primary target mRNA in a reversible and dynamic fashion, and formation of SgrS-mRNA complexes is rate-limiting, dictating the overall regulation efficiency in vivo. Examination of a secondary target indicated that differences in the target search kinetics contribute to setting the regulation priority among different target mRNAs.This super-resolution imaging and analysis approach provides a conceptual framework that can be generalized to other small RNA systems and other target search processes.
AB - Base-pairing interactions between nucleic acids mediate target recognition in many biological processes.We developed a super-resolution imaging and modeling platform that enabled the in vivo determination of base pairing-mediated target recognition kinetics.We examined a stress-induced bacterial small RNA, SgrS, which induces the degradation of target messenger RNAs (mRNAs). SgrS binds to a primary target mRNA in a reversible and dynamic fashion, and formation of SgrS-mRNA complexes is rate-limiting, dictating the overall regulation efficiency in vivo. Examination of a secondary target indicated that differences in the target search kinetics contribute to setting the regulation priority among different target mRNAs.This super-resolution imaging and analysis approach provides a conceptual framework that can be generalized to other small RNA systems and other target search processes.
UR - http://www.scopus.com/inward/record.url?scp=84925337335&partnerID=8YFLogxK
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U2 - 10.1126/science.1258849
DO - 10.1126/science.1258849
M3 - Article
C2 - 25792329
AN - SCOPUS:84925337335
SN - 0036-8075
VL - 347
SP - 1371
EP - 1374
JO - Science
JF - Science
IS - 6228
ER -