Abstract
A method for the permanent and simultaneous detection of tissue-specific surface antigens and internal radioactive labeling of mouse lymphoid cells is described. Target cells were first reacted with a mouse-derived 'antithymocyte serum', incubated with peroxidase-conjugated rabbit serum against mouse immunoglobulins and placed in substrate solution that leads to staining of the antigen-positive cells. Radioactive labeling was demonstrated by autoradiography performed after the antigen assay. More than 90% of antigen-positive thymocytes could be specifically stained in the assay without staining of similarly treated antigen-negative cells. Autoradiographic grains could be detected over both antigen-positive and antigen-negative cells.
Original language | English (US) |
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Pages (from-to) | 1269-1274 |
Number of pages | 6 |
Journal | Journal of Histochemistry and Cytochemistry |
Volume | 25 |
Issue number | 11 |
DOIs | |
State | Published - 1977 |
Externally published | Yes |
ASJC Scopus subject areas
- Anatomy
- Histology