TY - JOUR
T1 - Detection of somatic mutations and HPV in the saliva and plasma of patients with head and neck squamous cell carcinomas
AU - Wang, Yuxuan
AU - Springer, Simeon
AU - Mulvey, Carolyn L.
AU - Silliman, Natalie
AU - Schaefer, Joy
AU - Sausen, Mark
AU - James, Nathan
AU - Rettig, Eleni M.
AU - Guo, Theresa
AU - Pickering, Curtis R.
AU - Bishop, Justin A.
AU - Chung, Christine H.
AU - Califano, Joseph A.
AU - Eisele, David W.
AU - Fakhry, Carole
AU - Gourin, Christine G.
AU - Ha, Patrick K.
AU - Kang, Hyunseok
AU - Kiess, Ana
AU - Koch, Wayne M.
AU - Myers, Jeffrey N.
AU - Quon, Harry
AU - Richmon, Jeremy D.
AU - Sidransky, David
AU - Tufano, Ralph P.
AU - Westra, William H.
AU - Bettegowda, Chetan
AU - Diaz, Luis A.
AU - Papadopoulos, Nickolas
AU - Kinzler, Kenneth W.
AU - Vogelstein, Bert
AU - Agrawal, Nishant
PY - 2015/6/24
Y1 - 2015/6/24
N2 - To explore the potential of tumor-specific DNA as a biomarker for head and neck squamous cell carcinomas (HNSCC), we queried DNA from saliva or plasma of 93 HNSCC patients. We searched for somatic mutations or human papillomavirus genes, collectively referred to as tumor DNA. When both plasma and saliva were tested, tumor DNA was detected in 96% of 47 patients. The fractions of patients with detectable tumor DNA in earlyand late-stage disease were 100% (n = 10) and 95% (n = 37), respectively. When segregated by site, tumor DNA was detected in 100% (n = 15), 91% (n = 22), 100% (n = 7), and 100% (n = 3) of patients with tumors of the oral cavity, oropharynx, larynx, and hypopharynx, respectively. In saliva, tumor DNA was found in 100% of patients with oral cavity cancers and in 47 to 70% of patients with cancers of the other sites. In plasma, tumor DNA was found in 80% of patients with oral cavity cancers, and in 86 to 100% of patients with cancers of the other sites. Thus, saliva is preferentially enriched for tumor DNA from the oral cavity, whereas plasma is preferentially enriched for tumor DNA from the other sites. Tumor DNA in saliva was found postsurgically in three patients before clinical diagnosis of recurrence, but in none of the five patients without recurrence. Tumor DNA in the saliva and plasma appears to be a potentially valuable biomarker for detection of HNSCC.
AB - To explore the potential of tumor-specific DNA as a biomarker for head and neck squamous cell carcinomas (HNSCC), we queried DNA from saliva or plasma of 93 HNSCC patients. We searched for somatic mutations or human papillomavirus genes, collectively referred to as tumor DNA. When both plasma and saliva were tested, tumor DNA was detected in 96% of 47 patients. The fractions of patients with detectable tumor DNA in earlyand late-stage disease were 100% (n = 10) and 95% (n = 37), respectively. When segregated by site, tumor DNA was detected in 100% (n = 15), 91% (n = 22), 100% (n = 7), and 100% (n = 3) of patients with tumors of the oral cavity, oropharynx, larynx, and hypopharynx, respectively. In saliva, tumor DNA was found in 100% of patients with oral cavity cancers and in 47 to 70% of patients with cancers of the other sites. In plasma, tumor DNA was found in 80% of patients with oral cavity cancers, and in 86 to 100% of patients with cancers of the other sites. Thus, saliva is preferentially enriched for tumor DNA from the oral cavity, whereas plasma is preferentially enriched for tumor DNA from the other sites. Tumor DNA in saliva was found postsurgically in three patients before clinical diagnosis of recurrence, but in none of the five patients without recurrence. Tumor DNA in the saliva and plasma appears to be a potentially valuable biomarker for detection of HNSCC.
UR - http://www.scopus.com/inward/record.url?scp=84937611698&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84937611698&partnerID=8YFLogxK
U2 - 10.1126/scitranslmed.aaa8507
DO - 10.1126/scitranslmed.aaa8507
M3 - Article
C2 - 26109104
AN - SCOPUS:84937611698
SN - 1946-6234
VL - 7
JO - Science translational medicine
JF - Science translational medicine
IS - 293
M1 - 293ra104
ER -