TY - JOUR
T1 - Detection of proximal colorectal cancers through analysis of faecal DNA
AU - Traverso, Giovanni
AU - Shuber, Anthony
AU - Olsson, Louise
AU - Levin, Bernard
AU - Johnson, Constance
AU - Hamilton, Stanley R.
AU - Boynton, Kevin
AU - Kinzler, Kenneth W.
AU - Vogelstein, Bert
N1 - Funding Information:
This work was funded by the US National Colorectal Cancer Research Alliance, the Caroline Law Fund, the University of Texas M D Anderson Cancer Center, the Clayton Fund, and US National Institutes of Health grants CA 62924, CA 43460, and GM 07184. These funding sources had no role in the collection, analysis, or interpretation of data, in the writing of the report, or in the decision to submit the paper for publication.
PY - 2002/2/2
Y1 - 2002/2/2
N2 - Detection of mutations in faecal DNA represents a promising, non-invasive approach for detecting colorectal cancers in average-risk populations. One of the first practical applications of this technology involves the examination of microsatellite markers in sporadic cancers with mismatch-repair deficiencies. Since such cancers nearly always occur in the proximal colon, this test might be useful as an adjunct to sigmoidoscopy, which detects only distal colorectal lesions. We report here the first in-depth analysis of faecal DNA from patients with proximal cancers to determine the feasibility, sensitivity, and specificity of this approach. Using a sensitive method for microsatellite mutation detection, we found that 18 of 46 cancers had microsatellite alterations and that identical mutations could be identified in the faecal DNA of 17 of these 18 cases.
AB - Detection of mutations in faecal DNA represents a promising, non-invasive approach for detecting colorectal cancers in average-risk populations. One of the first practical applications of this technology involves the examination of microsatellite markers in sporadic cancers with mismatch-repair deficiencies. Since such cancers nearly always occur in the proximal colon, this test might be useful as an adjunct to sigmoidoscopy, which detects only distal colorectal lesions. We report here the first in-depth analysis of faecal DNA from patients with proximal cancers to determine the feasibility, sensitivity, and specificity of this approach. Using a sensitive method for microsatellite mutation detection, we found that 18 of 46 cancers had microsatellite alterations and that identical mutations could be identified in the faecal DNA of 17 of these 18 cases.
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U2 - 10.1016/S0140-6736(02)07591-8
DO - 10.1016/S0140-6736(02)07591-8
M3 - Article
C2 - 11844514
AN - SCOPUS:0037006377
SN - 0140-6736
VL - 359
SP - 403
EP - 404
JO - Lancet
JF - Lancet
IS - 9304
ER -