Detection of papillary thyroid carcinoma with serum protein profile analysis

William H. Moretz, Christine G. Gourin, David J. Terris, Zhong Sheng Xia, Zhongmin Liu, Paul M. Weinberger, Edward Chin, Bao Ling Adam

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Objective: To determine the sensitivity and specificity of surface-enhanced laser desorption/ionization time-offlight mass spectrometry (SELDI-TOF-MS) for papillary thyroid carcinoma (PTC) detection. Design: The SELDI-TOF-MS protein profiles of patients with PTC, patients with benign nodular disease (BND), and healthy controls were analyzed to determine the sensitivity and specificity of SELDI-TOF-MS assay for PTC detection. Data analysis was performed to process the spectral data and classify the disease status of the patients. Setting: Academic tertiary care hospital. Patients: Serum samples were collected prospectively from 7 patients with PTC, 8 patients with BND, and 7 healthy control volunteers. Intervention: All patients diagnosed as having PTC or BND underwent thyroidectomy from October 21, 2004, to January 31, 2006. Main Outcome Measures: Twenty-two serum samples were analyzed. Results: Most protein peaks resolved by the SELDI-TOF-MS assay were in the range of 1 to 20 kDa. Classification tree analysis based on peak expression distinguished patients with PTC from those with BND with 85.7% sensitivity and 100% specificity. Serum samples from patients with PTC differed most significantly from those of patients with BND by the underexpression of a protein peak at 11 101 Da. Conclusions: This pilot study demonstrates that proteomic analysis of serum protein profiles distinguishes patients with PTC from patients with BND with a high degree of sensitivity and specificity. Further investigation into the clinical utility of this technology in PTC biomarker detection and surveillance is warranted.

Original languageEnglish (US)
Pages (from-to)198-202
Number of pages5
JournalArchives of Otolaryngology--Head and Neck Surgery
Issue number2
StatePublished - Feb 2008
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology


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