Detection of Pancreatic Ductal Adenocarcinoma-Associated Proteins in Serum

T. Mamie Lih, Liwei Cao, Parham Minoo, Gilbert S. Omenn, Ralph H. Hruban, Daniel W. Chan, Oliver F. Bathe, Hui Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancer types, partly because it is frequently identified at an advanced stage, when surgery is no longer feasible. Therefore, early detection using minimally invasive methods such as blood tests may improve outcomes. However, studies to discover molecular signatures for the early detection of PDAC using blood tests have only been marginally successful. In the current study, a quantitative glycoproteomic approach via data-independent acquisition mass spectrometry was utilized to detect glycoproteins in 29 patient-matched PDAC tissues and sera. A total of 892 N-linked glycopeptides originating from 141 glycoproteins had PDAC-associated changes beyond normal variation. We further evaluated the specificity of these serum-detectable glycoproteins by comparing their abundance in 53 independent PDAC patient sera and 65 cancer-free controls. The PDAC tissue-associated glycoproteins we have identified represent an inventory of serum-detectable PDAC-associated glycoproteins as candidate biomarkers that can be potentially used for the detection of PDAC using blood tests.

Original languageEnglish (US)
Article number100687
JournalMolecular and Cellular Proteomics
Volume23
Issue number1
DOIs
StatePublished - Jan 2024

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Detection of Pancreatic Ductal Adenocarcinoma-Associated Proteins in Serum'. Together they form a unique fingerprint.

Cite this