Abstract
To substantiate reports of greater emergence of the K65R nucleoside reverse transcriptase inhibitor (NRTI) mutation in human immunodeficiency virus type 1 (HIV-1) subtype C, we examined natural low-level K65R expression in subtype C relative to subtypes B and AE. We used allele-specific polymerase chain reaction to screen HIV-1 amplified by reverse-transcription high-fidelity polymerase chain reaction from subtype C-infected South African women and infants and CRF01(subtype AE) from Thailand; all subjects were NRTI naive. We found low-level K65R of unknown clinical significance in NRTI-naive subtype C-infected women and infants at frequencies above the natural occurrence in subtypes B and AE. The frequent appearance of subtype C frameshift deletions at codon 65 supports a propensity for transcription error in this region.
Original language | English (US) |
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Pages (from-to) | 798-802 |
Number of pages | 5 |
Journal | Journal of Infectious Diseases |
Volume | 203 |
Issue number | 6 |
DOIs | |
State | Published - Mar 15 2011 |
Externally published | Yes |
ASJC Scopus subject areas
- Infectious Diseases
- Immunology and Allergy