Detection of individuals with acute HIV-1 infection using the ARCHITECT HIV Ag/Ab Combo assay

Susan H. Eshleman, Leila Khaki, Oliver Laeyendecker, Estelle Piwowar-Manning, Le Tanya Johnson-Lewis, Marla Husnik, Beryl Koblin, Thomas Coates, Margaret Chesney, Ana Vallari, Sushil G. Devare, John Hackett

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


BACKGROUND: We evaluated use of the ARCHITECT HIV Ag/Ab Combo assay (HIV Combo; Abbott Diagnostics; available for sale outside the United States only) for detection of acute HIV infection. METHODS: Samples were obtained from a behavioral intervention study (EXPLORE). HIV-uninfected men who have sex with men were enrolled and tested for HIV infection every 6 months. Samples from seroconverters collected at their last seronegative visit (n = 217) were tested individually using 2 HIV RNA assays. Samples with detectable HIV RNA were classified as acute and were tested with HIV Combo. Samples from the enrollment visit (n = 83) and the time of HIV seroconversion (n = 219) were tested with HIV Combo as controls. RESULTS: Twenty-one samples (9.7%) from the last seronegative visit had detectable HIV RNA and were classified as acute. HIV Combo was positive for 13 of the acute samples (61.9%). Samples not detected by HIV Combo had viral loads of 724-15,130 copies per milliliter. Expected results were obtained for positive and negative controls tested with HIV Combo. CONCLUSIONS: HIV Combo detected nearly two thirds of acute HIV infections identified in this high-risk population by non-pooled HIV RNA assays. HIV Combo may be useful for high-throughput screening to identify individuals with acute HIV infection.

Original languageEnglish (US)
Pages (from-to)121-124
Number of pages4
JournalJournal of Acquired Immune Deficiency Syndromes
Issue number1
StatePublished - Sep 2009


  • Acute infection
  • HIV Ag/Ab Combo assay
  • HIV-1

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)


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