Detection of human papovaviruses BKV and JCV in urines by ELISA.

R. R. Arthur; Keerti V Shah; R. H. Yolken; Patricia Charache

Detection of human papovaviruses BKV and JCV in urines by ELISA.

R. R. Arthur, Keerti V Shah, R. H. Yolken, Patricia Charache

School of Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A double-antibody indirect ELISA using a chromogenic substrate and a single-antibody sandwich direct ELISA using chromogenic and fluorogenic substrates were developed for the detection of the human papovaviruses BKV and JCV. All three assays were comparable in their sensitivity. BKV was detected in urine from 12 of 63 (19%) highly immunocompromised patients and JCV in urine from 4 of 31 (12.9%) patients by the indirect method.

Original language	English (US)
Pages (from-to)	169-176
Number of pages	8
Journal	Progress in clinical and biological research
Volume	105
State	Published - 1983

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Detection of human papovaviruses BKV and JCV in urines by ELISA.",

abstract = "A double-antibody indirect ELISA using a chromogenic substrate and a single-antibody sandwich direct ELISA using chromogenic and fluorogenic substrates were developed for the detection of the human papovaviruses BKV and JCV. All three assays were comparable in their sensitivity. BKV was detected in urine from 12 of 63 (19%) highly immunocompromised patients and JCV in urine from 4 of 31 (12.9%) patients by the indirect method.",

author = "Arthur, {R. R.} and Shah, {Keerti V} and Yolken, {R. H.} and Patricia Charache",

year = "1983",

language = "English (US)",

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pages = "169--176",

journal = "Progress in clinical and biological research",

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T1 - Detection of human papovaviruses BKV and JCV in urines by ELISA.

AU - Arthur, R. R.

AU - Shah, Keerti V

AU - Yolken, R. H.

AU - Charache, Patricia

PY - 1983

Y1 - 1983

N2 - A double-antibody indirect ELISA using a chromogenic substrate and a single-antibody sandwich direct ELISA using chromogenic and fluorogenic substrates were developed for the detection of the human papovaviruses BKV and JCV. All three assays were comparable in their sensitivity. BKV was detected in urine from 12 of 63 (19%) highly immunocompromised patients and JCV in urine from 4 of 31 (12.9%) patients by the indirect method.

AB - A double-antibody indirect ELISA using a chromogenic substrate and a single-antibody sandwich direct ELISA using chromogenic and fluorogenic substrates were developed for the detection of the human papovaviruses BKV and JCV. All three assays were comparable in their sensitivity. BKV was detected in urine from 12 of 63 (19%) highly immunocompromised patients and JCV in urine from 4 of 31 (12.9%) patients by the indirect method.

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ER -

Detection of human papovaviruses BKV and JCV in urines by ELISA.

Abstract

ASJC Scopus subject areas

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