Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine

Kamau Fahie, Bhargavi Narayanan, Fiddia Zahra, Russell Reeves, Steve M. Fernandes, Gerald W. Hart, Natasha E. Zachara

Research output: Contribution to journalArticlepeer-review


O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that regulates normal physiology and the cell stress response. Dysregulation of O-GlcNAc cycling is implicated in the etiology of type II diabetes, heart failure, hypertension, and Alzheimer's disease, as well as cardioprotection. These protocols cover simple and comprehensive techniques for detecting proteins modified by O-GlcNAc and studying the enzymes that add or remove O-GlcNAc.

Original languageEnglish (US)
Article numbere129
JournalCurrent Protocols
Issue number5
StatePublished - May 2021


  • O-GlcNAc
  • O-linked
  • analysis
  • detection
  • galactosyltransferase
  • glycosylation
  • signal transduction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Health Informatics
  • Medical Laboratory Technology
  • Medicine(all)


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